Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices
| Autor: | S. J. Hammad, S. D᾿ Amelio, Serena Cavallero, Fatima Shihab Al-Nasiri |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (General) Veterinary medicine Agriculture (General) 030231 tropical medicine Hydatid cyst morphometric study echinococcus granulosus S1-972 03 medical and health sciences Lactophenol blue R5-920 0302 clinical medicine parasitic diseases Echinococcus granulosus biology 030108 mycology & parasitology Proteinase K biology.organism_classification rostellar hook Metacestode protoscolices Morphometric analysis Lytic cycle biology.protein Animal Science and Zoology Parasitology |
| Zdroj: | Helminthologia, Vol 57, Iss 1, Pp 63-70 (2020) |
| ISSN: | 1336-9083 |
| Popis: | Summary Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue). |
| Databáze: | OpenAIRE |
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