Proton-irradiated breast cells: molecular points of view
| Autor: | Valentina Bravatà 1, Francesco P. Cammarata 1, Luigi Minafra 1, Pietro Pisciotta 1, 2, Concetta Scazzone 3, Lorenzo Manti 4, Gaetano Savoca 1, Giada Petringa 1, Giuseppe A.P. Cirrone 2, Giacomo Cuttone 2, Maria C. Gilardi 1, 5, Giusi I. Forte 1, Giorgio Russo 1 |
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| Přispěvatelé: | Bravata, V, Cammarata, F, Minafra, L, Pisciotta, P, Scazzone, C, Manti, L, Savoca, G, Petringa, G, Cirrone, G, Cuttone, G, Gilardi, M, Forte, G, Russo, G, Bravata, V., Cammarata, F. P., Minafra, L., Pisciotta, P., Scazzone, C., Manti, L., Savoca, G., Petringa, G., Cirrone, G. A. P., Cuttone, G., Gilardi, M. C., Forte, G. I., Russo, G., Bravatà, Valentina, Cammarata, Francesco P, Minafra, Luigi, Pisciotta, Pietro, Scazzone, Concetta, Manti, Lorenzo, Savoca, Gaetano, Petringa, Giada, Cirrone, Giuseppe A P, Cuttone, Giacomo, Gilardi, Maria C, Forte, Giusi I, Russo, Giorgio |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
breast cancer
cDNA microarray gene signature proton therapy radiation Breast Breast Neoplasms Cell Line Tumor DNA Complementary Dose-Response Relationship Radiation Female Gene Expression Profiling Gene Expression Regulation Neoplastic Humans Inflammation MCF-7 Cells Oligonucleotide Array Sequence Analysis Phenotype Proton Therapy Radiation Tolerance Radiotherapy Protons DNA Complementary Health Toxicology and Mutagenesis medicine.medical_treatment Breast Neoplasms Cell fate determination Radiation Tolerance gene signature 03 medical and health sciences 0302 clinical medicine Breast cancer breast cancer Cell Line Tumor Regular Paper medicine proton therapy Humans Radiology Nuclear Medicine and imaging Breast Clonogenic assay Biology Proton therapy Oligonucleotide Array Sequence Analysis 030304 developmental biology Inflammation cDNA microarray 0303 health sciences Radiotherapy Chemistry Gene Expression Profiling radiation Cancer Dose-Response Relationship Radiation Gene signature medicine.disease Gene Expression Regulation Neoplastic Gene expression profiling Radiation therapy Phenotype 030220 oncology & carcinogenesis MCF-7 Cells Cancer research Female Protons |
| Zdroj: | Journal of radiation research 60 (2019): 451–465. doi:10.1093/jrr/rrz032 info:cnr-pdr/source/autori:Valentina Bravatà 1*, Francesco P. Cammarata 1*, Luigi Minafra 1,* Pietro Pisciotta 1,2, Concetta Scazzone 3, Lorenzo Manti 4, Gaetano Savoca 1, Giada Petringa 1,2, Giuseppe A.P. Cirrone 2, Giacomo Cuttone 2, Maria C. Gilardi 1,5, Giusi I. Forte 1 and Giorgio Russo 1/titolo:Proton-irradiated breast cells: molecular points of view./doi:10.1093%2Fjrr%2Frrz032/rivista:Journal of radiation research/anno:2019/pagina_da:451/pagina_a:465/intervallo_pagine:451–465/volume:60 Journal of Radiation Research |
| Popis: | Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome |
| Databáze: | OpenAIRE |
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