Hyaluronan in human cerebrospinal fluid
| Autor: | Torvard C. Laurent, L. Persson, Ulla B.G. Laurent, M. Hartman, L. K. Hellsing, K Lilja |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Spinal Puncture Ventriculostomy chemistry.chemical_compound Cerebrospinal fluid Reference Values Seizures Hyaluronic acid medicine Humans Cyst Meningitis Hyaluronic Acid Child Aged Aged 80 and over Brain Diseases Cerebral infarction business.industry Brain Neoplasms Cysts Cartilage Infant General Medicine Cerebral Infarction Middle Aged medicine.disease Hydrocephalus Molecular Weight medicine.anatomical_structure Neurology chemistry Child Preschool Choroid plexus Female Neurology (clinical) business |
| Zdroj: | Acta neurologica Scandinavica. 94(3) |
| ISSN: | 0001-6314 |
| Popis: | We studied the concentration of hyaluronan in cerebrospinal fluid (CSF) in various diseases and attempted to define its reference interval. A radioassay utilizing cartilage proteins with affinity for hyaluronan was used in determining the concentration of 200 lumbar and 27 ventricular CSF specimens and 11 brain cyst fluids. Molecular weight distributions were determined by gel chromatography and localization in brain tissue by histochemistry. The hyaluronan level of lumbar CSF showed an increase with age; comparatively healthy children had (mean +/- SD) 50 +/- 41 micrograms/L (n = 40) and adults 166 +/- 77 micrograms/L (n = 9); i.e. significantly different values. The highest level was recorded in a patient with meningitis (> 8000 micrograms/L). More than 4000 micrograms/ L was noted in a patient with tumour metastasis in the cerebellum. Significantly elevated levels were especially found with spinal stenosis, head injury and cerebral infarction, but also in inflammatory medical disorders, hydrocephalus and encephalitis. We found no significant increase in multiple sclerosis and some other neurological diseases. Ventricular CSF of adults contained significantly less hyaluronan (53 +/- 73 micrograms/L; n = 16) than lumbar CSF. Hyaluronan in cyst fluids varied from 31 to 25,000 micrograms/L. Weight average molecular weight of hyaluronan in CSF was 2.9-3.0 x 10(5) and in brain tumour cyst fluid 2.4 x 10(6). In search for the origin of hyaluronan in CSF it was found that its concentration in the choroid plexus and leptomeninges was low, but that hyaluronan was accumulated in the superficial layer of the cerebral cortex. Continued screening for hyaluronan in CSF may be valuable in cases of inflammatory diseases, tumours and obstruction to CSF flow. |
| Databáze: | OpenAIRE |
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