HIV-1 Gag p24-Nef fusion peptide induces cellular and humoral immune response in a mouse model
| Autor: | Mehdi Mahdavi, Massoumeh Ebtekar, Fatemeh Rahbarizadeh, Kayhan Azadmanesh, Zuhair Mohammad Hassan, Mohammad Reza Hairi Yazdi, Khorramkhorshid Hr, Rezvan Zabihollahi, Mohsen Abolhassani |
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| Rok vydání: | 2010 |
| Předmět: |
Recombinant Fusion Proteins
HIV Core Protein p24 chemical and pharmacologic phenomena Lymphocyte proliferation HIV Antibodies Lymphocyte Activation Interferon-gamma Mice Immune system Virology Cytotoxic T cell Animals Humans nef Gene Products Human Immunodeficiency Virus HIV vaccine Antibody-dependent cell-mediated cytotoxicity AIDS Vaccines Immunity Cellular Mice Inbred BALB C biology virus diseases General Medicine Acquired immune system Immunity Humoral CTL Infectious Diseases Immunoglobulin G Immunology Models Animal biology.protein HIV-1 Female Interleukin-4 Antibody T-Lymphocytes Cytotoxic |
| Zdroj: | Acta virologica. 54(2) |
| ISSN: | 0001-723X |
| Popis: | UNLABELLED Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-gamma and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided. KEYWORDS epitopes; fusion peptide; HIV-1 p24-Nef; immune response. |
| Databáze: | OpenAIRE |
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