Simultaneous determination of dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F 490), in plasma and urine of man and dog by high-performance liquid chromatography

Autor:	E. van der Kleyn, A.C.L.M. Grutters, E.W. Wuis, Tom B. Vree
Rok vydání:	1982
Předmět:	Male Detection limit Chromatography Chemistry Extraction (chemistry) Skeletal muscle General Chemistry Urine High-performance liquid chromatography Dantrolene Dogs medicine.anatomical_structure Pharmacokinetics Nitro medicine Animals Humans Chromatography High Pressure Liquid medicine.drug
Zdroj:	Journal of Chromatography B: Biomedical Sciences and Applications. 231:401-409
ISSN:	0378-4347
DOI:	10.1016/s0378-4347(00)81864-2
Popis:	A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of the skeletal muscle relaxant dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F-490), in plasma and urine of man and dog. The substances are detected spectrophotometrically at 375 nm. The detection limits are 0.02 mg/l. A preliminary extraction step into a choloroform-butanol mixture is required for the plasma samples. The method is suitable for pharmacokinetic studies of dantrolene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::671a683bb47133ecdb3d7b76b23baeb2 https://doi.org/10.1016/s0378-4347(00)81864-2 Zobrazit plný text záznamu