Antibodies to Murine CD40 Stimulate Normal B Lymphocytes but Inhibit Proliferation of B Lymphoma Cells
| Autor: | Edward A. Clark, Andrew W. Heath, Charles Hannum, David Campbell, Ray Chang, Nobuyuki Harada, Raul M. Torres, Leopoldo Santos-Argumedo, Maureen Howard, John R. Gordon, Armen B. Shanafelt |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lymphoma
B-Cell medicine.drug_class Molecular Sequence Data Immunology Cell Moths Lymphocyte Activation Monoclonal antibody Cell Line law.invention Mice Antigens CD law medicine Extracellular Animals CD40 Antigens Antiserum B-Lymphocytes Mice Inbred BALB C CD40 Base Sequence biology Cell growth Immune Sera hemic and immune systems Molecular biology Recombinant Proteins Rats Antigens Differentiation B-Lymphocyte medicine.anatomical_structure Solubility Rats Inbred Lew biology.protein Recombinant DNA Female Antibody Cell Division |
| Zdroj: | Cellular Immunology. 152:468-480 |
| ISSN: | 0008-8749 |
| DOI: | 10.1006/cimm.1993.1305 |
| Popis: | A rat anti-mouse CD40 antiserum has been prepared by hyperimmunisation of Lewis rats with a highly purified preparation of the recombinant extracellular domain of murine CD40. This antiserum specifically binds CD40-expressing L cell transfectants, but not untransfected L cells, and induces vigorous proliferation of highly purified small dense B cells obtained from the spleens of unstimulated mice. Anti-CD40-induced B cell proliferation can be augmented by the addition of IL-4 and is inhibited by purified recombinant soluble mouse CD40. Interestingly the same anti-CD40 antiserum specifically inhibits the in vitro growth of A.20 murine B lymphoma cells. The specificity of this inhibition can be demonstrated by reversing the effect with purified recombinant soluble mouse CD40. These data implicate CD40 as a possible target for therapeutic intervention in the treatment of B lymphomas. |
| Databáze: | OpenAIRE |
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