Evaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine

Autor: Erdal Bedir, Ayse Nalbantsoy, Nusin Cebi, Nejdet Coven, Furkan Ozan Coven, Rükan Genç, Nilgun Yakubogullari, Fethiye Coven
Rok vydání: 2021
Předmět:
0301 basic medicine
medicine.medical_treatment
Saponin
Antibodies
Viral
Applied Microbiology and Biotechnology
Mice
Squalene
chemistry.chemical_compound
0302 clinical medicine
Qs-21
030212 general & internal medicine
Neutralizing antibody
chemistry.chemical_classification
Natural products
biology
Viral Vaccine
General Medicine
Triterpene Glycosides
Triterpenoid saponin
Antibody
Adjuvant
Biotechnology
Newcastle Disease
Newcastle disease virus
Bioengineering
Vaccines
Attenuated
Vaccine adjuvant
Interferon-gamma
03 medical and health sciences
Immune system
Adjuvants
Immunologic
Polysaccharides
Splenocyte
medicine
Animals
Pharmacology
General Immunology and Microbiology
business.industry
Viral Vaccines
Saponins
Antibodies
Neutralizing
Roots
Virology
Astragalus polysaccharide
030104 developmental biology
Vaccines
Inactivated
chemistry
In-Vitro
biology.protein
Interleukin-2
business
Astragaloside VII
Zdroj: Biologicals. 70:28-37
ISSN: 1045-1056
Popis: Astragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants. © 2021 International Alliance for Biological Standardization
1139B411402292 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK
This study partially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under Grant 1139B411402292 .
Databáze: OpenAIRE
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