Colony counting is a major source of variation in CFU-GM results between centres

Autor:	Matthew Armstrong Lumley, Robert Burgess, Donald Milligan, Lucinda Billingham, Dorothy McDonald
Rok vydání:	1997
Předmět:	Colony-forming unit Veterinary medicine Analysis of Variance Macrophages CFU-GM Significant difference Hematology Hematologic Neoplasms Biology Hematopoietic Stem Cells Colony-Forming Units Assay Multicenter study Reference Values Reference values Colony count Humans Colony counting Granulocytes
Zdroj:	British journal of haematology. 97(2)
ISSN:	0007-1048
Popis:	The results for colony forming unit granulocyte-macrophage (CFU-GM) assays vary substantially between centres. It is possible that colony counting is largely responsible for this discrepancy. In order to examine this exclusively from the many factors that make up the CFU-GM assay, we performed a colony counting exercise involving 11 laboratories. Two-way analysis of variance showed a highly significant difference (P = 0.0001) in the counts obtained from the centres. One centre was found to score consistently high and two others scored consistently low numbers of colonies. This suggests that identification of colonies is a major source of variation between centres.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67154cd12d97c68d89c8888bc8bfbe08 https://pubmed.ncbi.nlm.nih.gov/9163619 Zobrazit plný text záznamu Plný text