Lišće biljke Ficus deltoidea utječe na oksidacijski stres, homeostazu proteina i ubikvitin-proteasom sustav u jetrenim stanicama izloženim djelovanju masnih kiselina

Autor: Noor Nazirahanie Abrahim, Norhaniza Aminudin, Puteri Shafinaz Abdul-Rahman
Rok vydání: 2023
Předmět:
Zdroj: Food Technology and Biotechnology
Volume 61
Issue 2
ISSN: 1334-2606
1330-9862
Popis: Research background. Ficus deltoidea (mistletoe fig) is a shrub well known among locals in Malaysia primarily for its treatment of toothaches, colds and wounds. The aim of this study is to determine the potential of leaves, sourced from three different varieties of F. deltoidea, to exhibit antioxidant activity, a reduction of lipid concentration, and protein expression in steatosis-induced liver cell lines. Experimental approach. The leaves of three F. deltoidea varieties, namely Ficus deltoidea var. angustifolia, Ficus deltoidea var. trengganuensis and Ficus deltoidea var. kunstleri, were subjected to water extraction. The resulting crude extracts were fractionated using water and ethyl acetate. Palmitic acid was used to induce lipid accumulation (steatosis) in human liver (WRL68) cells, before all the samples were tested for their lipid-reducing activity. Several proteomic approaches were incorporated. The changes in protein expression were determined using 2-dimensional gel electrophoresis separation, whereas identification of our protein spots of interest was carried out via matrix-assisted laser desorption/ionization time-of-flight. Results and conclusions. Ficus deltoidea var. kunstleri alone demonstrated the ability to reduce lipids at the highest tested concentration (200 µg/mL) and was, therefore, used for subsequent experiments. Treatment with Ficus deltoidea var. kunstleri was found to restore redox status by increasing superoxide dismutase and glutathione peroxidase amounts and decreasing malondialdehyde formation. Six proteins were successfully identified; these were heat shock protein beta-1 (HSPB1), proteasome subunit alpha type 1 (PSMA1), glutathione S-transferase omega 1 (GSTO1), peroxiredoxin-1 (PRDX1), histone H2B (HIST1H2BD) and ubiquitin c-terminal hydrolase L3 (UCHL3). Through bioinformatics analysis, it was found that these proteins were significantly involved in specific pathways such as oxidative stress (PRDX1 and GSTO1), protein homeostasis (HSPB1) and degradation (UCHL3 and PSMA1). Novelty and scientific contribution. F. deltoidea pretreatment was shown to reduce lipid accumulation, thus improving the redox status and protein homeostasis. This suggests the role of F. deltoidea as a preventive mechanism in non-alcohol fatty liver disease.
Pozadina istraživanja. Ficus deltoidea (sitnolisni fikus) je grmolika biljka koju stanovnici Malezije dobro poznaju, jer ju koriste prvenstveno za liječenje zubobolje, prehlade i rana. Svrha je ovoga rada bila odrediti sposobnost ekstrakata lišća triju sorata biljke F. deltoidea da ispolje antioksidacijsku aktivnost, smanje nakupljanje lipida i reguliraju ekspresiju proteina u stanicama masne jetre. Eksperimentalni pristup. Ekstrakcija lišća triju sorata biljke F. deltoidea, i to Ficus deltoidea var. angustifolia, Ficus deltoidea var. trengganuensis i Ficus deltoidea var. kunstleri, provedena je u vodenoj otopini. Od sirovog ekstrakta lišća dobivene su frakcije pomoću vode i etil-acetata. Palmitinska kiselina je upotrijebljena za poticanje steatoze (nakupljanja lipida) u stanicama jetre čovjeka (WRL68), a zatim je ispitana sposobnost ekstrakata da smanje količinu lipida. Primijenjeno je nekoliko proteomičkih pristupa. Promjene u ekspresiji proteina određene su pomoću dvodimenzionalne gel elektroforeze, dok su proteini od interesa identificirani MALDI-ToF masenom spektrometrijom. Rezultati i zaključci. Jedino je biljka sorte Ficus deltoidea var. kunstleri imala sposobnost smanjivanja akumulacije lipida pri najvećoj ispitanoj koncentraciji (200 μg/mL), te je stoga korištena u daljnjim ispitivanjima. Obradom ekstraktom biljke F. deltoidea var. kunstleri obnovljen je redoks status stanica uslijed povećanja količine superoksid dismutaze i smanjenja proizvodnje malondialdehida. Uspješno je identificirano šest proteina: HSPB1, PSMA1, GSTO1, PRDX1, HIST1H¬2BD i UCHL3. Bioinformatička je analiza pokazala bitnu ulogu ovih proteina u specifičnim putevima kao što su oksidacijski stres (PRDX1 i GSTO1), homeostaza proteina (HSPB1) i njihova razgradnja (UCHL3 i PSMA1). Novina i znanstveni doprinos. Prethodnom obradom stanica jetre ekstraktom biljke F. deltoidea smanjilo se nakupljanje lipida, čime su poboljšani redoks status i homeostaza proteina u stanicama. Stoga se može zaključiti da biljka F. deltoidea ima ulogu u sprečavanju razvoja nealkoholne bolesti jetre.
Databáze: OpenAIRE
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