Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
| Autor: | Luciana Maria Silva, Gustavo Ferreira Martins, Paulo F. P. Pimenta, Consuelo Latorre Fortes-Dias, José Marcelo Ramalho-Ortigão, José Eduardo Serrão, B.A.M. Guedes |
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| Rok vydání: | 2011 |
| Předmět: |
Morphology
0106 biological sciences Cell type Embryo Nonmammalian Cell Survival media_common.quotation_subject Cell Culture Techniques Aedes aegypti Insect Biology 01 natural sciences 03 medical and health sciences Mosquito Aedes Animals Cells Cultured Primary culture 030304 developmental biology media_common 0303 health sciences Oenocyte fungi Pupa Embryo Cell Biology General Medicine Isolation (microbiology) biology.organism_classification Virology In vitro 3. Good health Cell biology 010602 entomology Cell culture Microscopy Electron Scanning Female Developmental Biology |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2010.12.003 |
| Popis: | Oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells with a smooth surface. Our results demonstrate that these cells remain viable in cell culture for at least two months. We also investigated their morphology in vivo and in vitro using light, confocal, scanning and transmission electron microscopes. This work is the first successful attempt in isolating and maintaining Ae. aegypti oenocytes in culture, and a significant step towards understanding the role of this cell type in this important disease vector. The purification and the development of primary cultures of insect oenocytes will allow future studies of their metabolism in producing and secreting compounds. |
| Databáze: | OpenAIRE |
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