DSG3 as a biomarker for the ultrasensitive detection of occult lymph node metastasis in oral cancer using nanostructured immunoarrays
| Autor: | Christina A. Marsh, Ruchika Malhotra, Daniel Martin, Vyomesh Patel, Bhuvanesh Singh, Timothy D. Veenstra, Uttam K. Sinha, Alfredo A. Molinolo, James F. Rusling, Cherie-Ann O. Nathan, Colleen L. Doçi, J. Silvio Gutkind |
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| Rok vydání: | 2012 |
| Předmět: |
Oncology
Cancer Research medicine.medical_specialty Pathology Blotting Western Microfluidics Sensitivity and Specificity Article stomatognathic system Internal medicine otorhinolaryngologic diseases Carcinoma Biomarkers Tumor Medicine Humans education Lymph node Mouth neoplasm education.field_of_study Desmoglein 3 business.industry Cancer medicine.disease Head and neck squamous-cell carcinoma Immunohistochemistry Nanostructures stomatognathic diseases medicine.anatomical_structure Cervical lymph nodes Tissue Array Analysis Lymphatic Metastasis Carcinoma Squamous Cell Mouth Neoplasms Lymph Oral Surgery business |
| Zdroj: | Oral oncology. 49(2) |
| ISSN: | 1879-0593 |
| Popis: | Summary Objectives The diagnosis of cervical lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) patients constitutes an essential requirement for clinical staging and treatment selection. However, clinical assessment by physical examination and different imaging modalities, as well as by histological examination of routine lymph node cryosections can miss micrometastases, while false positives may lead to unnecessary elective lymph node neck resections. Here, we explored the feasibility of developing a sensitive assay system for desmoglein 3 (DSG3) as a predictive biomarker for lymph node metastasis in HNSCC. Materials and methods DSG3 expression was determined in multiple general cancer- and HNSCC-tissue microarrays (TMAs), in negative and positive HNSCC metastatic cervical lymph nodes, and in a variety of HNSCC and control cell lines. A nanostructured immunoarray system was developed for the ultrasensitive detection of DSG3 in lymph node tissue lysates. Results We demonstrate that DSG3 is highly expressed in all HNSCC lesions and their metastatic cervical lymph nodes, but absent in non-invaded lymph nodes. We show that DSG3 can be rapidly detected with high sensitivity using a simple microfluidic immunoarray platform, even in human tissue sections including very few HNSCC invading cells, hence distinguishing between positive and negative lymph nodes. Conclusion We provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis and guide in the selection of appropriate therapeutic intervention modalities for HNSCC patients. |
| Databáze: | OpenAIRE |
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