Assay for ubiquitin ligase activity: high-throughput screen for inhibitors of HDM2

Autor:	Allan M. Weissman, Pankaj Oberoi, Howard O. Fearnhead, Shengyun Fang, D. Woods, Jane P. Jensen, Yassamin J. Safiran, J. H. Kenten, Karen H. Vousden, Ilia Davydov
Rok vydání:	2005
Předmět:	0301 basic medicine Ubiquitin-Protein Ligases Drug Evaluation Preclinical Biochemistry Analytical Chemistry 03 medical and health sciences Mice 0302 clinical medicine Ubiquitin Proto-Oncogene Proteins Animals Combinatorial Chemistry Techniques Humans Enzyme Inhibitors Ubiquitins biology Ubiquitin ligase activity Chemistry Nuclear Proteins Proto-Oncogene Proteins c-mdm2 Molecular biology Ubiquitin ligase Protein Structure Tertiary 030104 developmental biology Spectrometry Fluorescence 030220 oncology & carcinogenesis biology.protein Molecular Medicine Mdm2 Biological Assay Biotechnology
Zdroj:	Journal of biomolecular screening. 9(8)
ISSN:	1087-0571
Popis:	An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput format to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::670d95a7a844be971c95fb29f22d6ad6 https://pubmed.ncbi.nlm.nih.gov/15634796 Zobrazit plný text záznamu