Molecular Target Therapy of AKT and NF-kB Signaling Pathways and Multidrug Resistance by Specific Cell Penetrating Inhibitor Peptides in HL-60 Cells
| Autor: | Zohre Sadeghi, Mohsen Alipour, Zahra Davoudi, Nosratollah Zarghami, Ali Akbar Movassaghpour, Kazem Nejati-Koshki, Abolfazl Akbarzadeh, Hassan Dariushnejad, Mohammad Rahmati-Yamchi |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
ATP Binding Cassette Transporter Subfamily B Myeloid Cell Survival Epidemiology Cell Apoptosis HL-60 Cells Cell-Penetrating Peptides Biology Cell Line Tumor medicine Humans RNA Messenger Viability assay Protein kinase B Cell Proliferation Phosphoinositide-3 Kinase Inhibitors NF-kappa B Public Health Environmental and Occupational Health Myeloid leukemia medicine.disease Molecular biology Drug Resistance Multiple Leukemia Myeloid Acute Leukemia medicine.anatomical_structure Oncology Drug Resistance Neoplasm Cell culture Signal transduction Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 15:4353-4358 |
| ISSN: | 1513-7368 |
| Popis: | Background: PI3/AKT and NF-kB signaling pathways are constitutively active in acute myeloid leukemia and cross-talk between the two has been shown in various cancers. However, their role in acute myeloid leukemia has not been completely explored. We therefore used cell penetrating inhibitor peptides to define the contributions of AKT and NF-kB to survival and multi drug resistance (MDR) in HL-60 cells. Materials and Methods: Inhibition of AKT and NF-kB activity by AKT inhibitor peptide and NBD inhibitor peptide, respectively, resulted in decreased expression of mRNA for the MDR1 gene as assessed by real time PCR. In addition, treatment of HL-60 cells with AKT and NBD inhibitor peptides led to inhibition of cell viability and induction of apoptosis in a dose dependent manner as detected by flow cytometer. Results: Finally, co-treatment of HL-60 cells with sub-optimal doses of AKT and NBD inhibitor peptides led to synergistic apoptotic responses in AML cells. Conclusions: These data support a strong biological link between NF-kB and PI3-kinase/AKT pathways in the modulation of antiapoptotic and multi drug resistant effects in AML cells. Synergistic targeting of these pathways using NF-kB and PI3-kinase/AK inhibitor peptides may have a therapeutic potential for AML and possibly other malignancies with constitutive activation of these pathways. |
| Databáze: | OpenAIRE |
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