Attenuated PGC-1α Isoforms following Endurance Exercise with Blood Flow Restriction
| Autor: | Sara Serag, Cláudia Regina Cavaglieri, Arthur Fernandes Gáspari, Miguel S. Conceição, Felipe Cassaro Vechin, Patricia Chakur Brum, Edson Manoel Mendes Junior, Mara Patricia Traina Chacon-Mikahil, Bruce M. Spiegelman, Donny M. Camera, Cleiton Augusto Libardi, John A. Hawley, André L. L Andrade, Guilherme Defante Telles |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A mitochondrial biogenesis Angiogenesis Muscle Proteins adaptation Tripartite Motif Proteins RC1200 angiogenesis 0302 clinical medicine Protein Isoforms Orthopedics and Sports Medicine Leg press high-intensity exercise Cross-Over Studies medicine.diagnostic_test Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha medicine.anatomical_structure Adult Peroxisome proliferator-activated receptor gamma medicine.medical_specialty Ubiquitin-Protein Ligases Physical Therapy Sports Therapy and Rehabilitation 03 medical and health sciences Young Adult Oxygen Consumption Endurance training Internal medicine medicine Humans cell signaling Exercise physiology skeletal muscle Muscle Skeletal Exercise Muscle biopsy business.industry EXERCÍCIO FÍSICO Skeletal muscle Resistance Training 030229 sport sciences Hypoxia-Inducible Factor 1 alpha Subunit 030104 developmental biology Endocrinology Mitochondrial biogenesis Regional Blood Flow Exercise Test Physical Endurance business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1530-0315 |
| Popis: | Introduction Exercise performed with blood flow restriction simultaneously enhances the acute responses to both myogenic and mitochondrial pathways with roles in training adaptation. We investigated isoform-specific gene expression of the peroxisome proliferator-activated receptor gamma coactivator 1 and selected target genes and proteins regulating skeletal muscle training adaptation. Methods Nine healthy, untrained males participated in a randomized, counterbalanced, crossover design in which each subject completed a bout of low-intensity endurance exercise performed with blood flow restriction (15 min cycling at 40% of V˙O2peak, BFR-EE), endurance exercise (30 min cycling at 70% of V˙O2peak, EE), or resistance exercise (4 × 10 repetitions of leg press at 70% of one-repetition maximum) separated by at least 1 wk of recovery. A single resting muscle biopsy (vastus lateralis) was obtained 2 wk before the first exercise trial (rest) and 3 h after each bout. Results Total PGC-1α mRNA abundance, along with all four isoforms, increased above rest with EE only (P < 0.05) being higher than BFR-EE (P < 0.05). PGC-1α1, 2, and 4 were higher after EE compared with resistance exercise (P < 0.05). EE also increased vascular endothelial growth factor, Hif-1α, and MuRF-1 mRNA abundance above rest (P < 0.05), whereas COXIV mRNA expression increased with EE compared with BFR-EE (P < 0.05). Conclusion The attenuated expression of all four PGC-1α isoforms when EE is performed with blood flow restriction suggests this type of exercise provides an insufficient stimulus to activate the signaling pathways governing mitochondrial and angiogenesis responses observed with moderate- to high-intensity EE. |
| Databáze: | OpenAIRE |
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