Experimental and field investigations of exposure, replication and transmission of SARS-CoV-2 in pigs in the Netherlands
| Autor: | Sikkema, Reina S, Tobias, Tijs, Oreshkova, Nadia, de Bruin, Erwin, Okba, Nisreen, Chandler, Felicity, Hulst, Marcel M, Rodon, Jordi, Houben, Manon, van Maanen, Kees, Bultman, Hans, Meester, Marina, Gerhards, Nora M, Bouwknegt, Martijn, Urlings, Bert, Haagmans, Bart, Kluytmans, Jan, Geurtsvan Kessel, Corine H, van der Poel, Wim H M, Koopmans, Marion P G, Stegeman, Arjan, FAH GZ varken, dFAH AVR, dFAH I&I, LS Virologie, FAH veterinaire epidemiologie |
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| Přispěvatelé: | Producció Animal, Sanitat Animal, FAH GZ varken, dFAH AVR, dFAH I&I, LS Virologie, FAH veterinaire epidemiologie, Virology |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Letter
Bioinformatica & Diermodellen Coronaviruses Epidemiology Swine Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Infectious and parasitic diseases RC109-216 Biology medicine.disease_cause Microbiology SDG 3 - Good Health and Well-being Virology antibody Bio-informatics & Animal models Drug Discovery Replication (statistics) medicine Animals Public Health Surveillance Epidemiology Bio-informatics & Animal models One Health Animal species Netherlands Retrospective Studies Coronavirus Epidemiologie Swine Diseases Transmission (medicine) SARS-CoV-2 fungi COVID-19 food and beverages Environmental Exposure General Medicine QR1-502 Virology & Molecular Biology Virologie & Moleculaire Biologie Infectious Diseases Epidemiologie Bioinformatica & Diermodellen Parasitology |
| Zdroj: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Emerging Microbes and Infections, 11(1), 91-94 Emerging Microbes & Infections article-version (VoR) Version of Record Emerging Microbes and Infections, 11(1), 91-94. Nature Publishing Group Emerging Microbes and Infections 11 (2022) 1 Emerging Microbes and Infections, Vol 11, Iss 1, Pp 91-94 (2022) Emerging Microbes and Infections, 11(1), 91. Nature Publishing Group |
| ISSN: | 2222-1751 |
| Popis: | In order to assess the risk of SARS-CoV-2 infection, transmission and reservoir development in swine, we combined results of an experimental and two observational studies. First, intranasal and intratracheal challenge of eight pigs did not result in infection, based on clinical signs and PCR on swab and lung tissue samples. Two serum samples returned a low positive result in virus neutralization, in line with findings in other infection experiments in pigs. Next, a retrospective observational study was performed in the Netherlands in the spring of 2020. Serum samples (N =417) obtained at slaughter from 17 farms located in a region with a high human case incidence in the first wave of the pandemic. Samples were tested with protein micro array, plaque reduction neutralization test and receptor-binding-domain ELISA. None of the serum samples was positive in all three assays, although six samples from one farm returned a low positive result in PRNT (titers 40-80). Therefore we conclude that serological evidence for large scale transmission was not observed. Finally, an outbreak of respiratory disease in pigs on one farm, coinciding with recent exposure to SARS-CoV-2 infected animal caretakers, was investigated. Tonsil swabs and paired serum samples were tested. No evidence for infection with SARS-CoV-2 was found. In conclusion, Although in both the experimental and the observational study few samples returned low antibody titer results in PRNT infection with SARS-CoV-2 was not confirmed. It was concluded that sporadic infections in the field cannot be excluded, but large-scale SARS-CoV-2 transmission among pigs is unlikely. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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