Principles and Practices of Laser Scanning Confocal Microscopy
| Autor: | Stephen W. Paddock |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Materials science Microscope Laser scanning Confocal Fluorescent Antibody Technique Bioengineering Image processing Applied Microbiology and Biotechnology Biochemistry law.invention Optics Optical microscope law Confocal microscopy Microscopy Image Processing Computer-Assisted Medical imaging Animals Wings Animal Molecular Biology Fluorescent Dyes Cell Nucleus Microscopy Confocal business.industry Drosophila business Butterflies Software Biotechnology |
| Zdroj: | Molecular Biotechnology. 16:127-150 |
| ISSN: | 1073-6085 |
| DOI: | 10.1385/mb:16:2:127 |
| Popis: | The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today's sophisticated instruments are outlined. The major imaging modes of the LSCM are introduced including single optical sections, multiple wavelength images, three-dimensional reconstructions, and living cell and tissue sequences. Practical aspects of specimen preparation, image collection, and image presentation are included along with a primer on troubleshooting the LSCM for the novice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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