Enzyme, protein, carbohydrate, and phenolic contaminants in commercial tyrosinase preparations: potential problems affecting tyrosinase activity and inhibition studies
| Autor: | William H. Flurkey, Akhila Reddy, Kelli Spoonmore, Jena Cooksey, Antonio Rescigno, Jennifer K. Inlow, Allison Flurkey |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Laccase biology Monophenol Monooxygenase Tyrosinase Agaricus Carbohydrates Proteins General Chemistry Cellulase Enzyme assay Enzymes Enzyme chemistry Biochemistry Phenols Chitinase biology.protein Xylanase Enzyme Inhibitors General Agricultural and Biological Sciences Drug Contamination Agaricus bisporus |
| Zdroj: | Journal of agricultural and food chemistry. 56(12) |
| ISSN: | 1520-5118 |
| Popis: | Commercial mushroom tyrosinase contains other proteins, enzymes, carbohydrates, and phenolic material besides tyrosinase. Carbohydrate and phenolic material comprise a large percentage of the powder resuspensions derived from Agaricus bisporus. Enzyme assays identified the presence of tyrosinase, laccase, beta-glucosidase, beta-galactosidase, beta-xylosidase, cellulase, chitinase, xylanase, and mannanase in the commercial tyrosinase. Protein sequencing indicated the presence of tyrosinase, a lectin, and a putative mannanase as well as 10 unidentified protein/peptides in the commercial tyrosinase preparations. Characteristics of tyrosinase isoforms were similar in two different commercial tyrosinase sources. Inhibition studies indicated that I 50 values for some tyrosinase inhibitors were different when the crude powder was compared to a partially purified tyrosinase. The presence of these contaminants has the potential to affect studies using commercial tyrosinase. |
| Databáze: | OpenAIRE |
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