Analysis of 5-bromouracil distribution in partially substituted deoxyribonucleic acids

Autor:	John L. Couch, Philip C. Hanawalt
Rok vydání:	1971
Předmět:	5-Bromouracil DNA Bacterial Bromouracil biology Biophysics Phosphorus Isotopes Cell Biology DNA biology.organism_classification Biochemistry Thymine chemistry.chemical_compound chemistry Molar ratio Methods Thymine Nucleotides Chromatography Thin Layer Uracil Molecular Biology Bacteria
Zdroj:	Analytical biochemistry. 41(1)
ISSN:	0003-2697
Popis:	A method has been developed for quantitatively estimating the contribution of pools of thymine derivatives to the DNA synthesized by bacteria grown in the presence of the analog 5-bromouracil. A 3′-nucleotide digest of the DNA synthesized during a period of labeling with 32P and 5BU is resolved by thin-layer chromatography to determine the 5BU T molar ratio in the newly synthesized DNA. The method is applicable to the quantitative estimation of turnover and repair in the DNA of growing cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6702e29c4975396ff9dccb33de6e0ce3 https://pubmed.ncbi.nlm.nih.gov/5578553 Zobrazit plný text záznamu