A new ultra-high pressure liquid chromatography method for the determination of antioxidant flavonol aglycones in six Lysimachia species
Autor: | Szabolcs Béni, Krisztina Végh, Ágnes Kéry, Ágnes Alberti, Anita Tóth |
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Rok vydání: | 2016 |
Předmět: |
Flavonols
Lysimachia ciliata Plant Science 01 natural sciences Biochemistry Antioxidants Analytical Chemistry Lysimachia vulgaris Lysimachia clethroides chemistry.chemical_compound Kaempferols Chromatography High Pressure Liquid Primulaceae Flavonoids Chromatography biology 010405 organic chemistry Plant Extracts Organic Chemistry Free Radical Scavengers biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Lysimachia Myricetin Lysimachia punctata Quercetin Kaempferol |
DOI: | 10.6084/m9.figshare.3189112.v1 |
Popis: | UPLC-DAD method was developed and validated for the quantitative determination of free flavonol aglycones (kaempferol, quercetin and myricetin) after acidic hydrolysis in six Lysimachia species. Quantitative analyses showed that the amounts of various flavonol aglycones were significantly different in Lysimachia vulgaris, Lysimachia nummularia, Lysimachia punctata, Lysimachia christinae, Lysimachia ciliata and Lysimachia clethroides. The L. clethroides sample was found to be the richest in kaempferol (25.77 ± 1.29 μg/mg extract) and quercetin (97.67 ± 4.61 μg/mg extract), while the L. nummularia sample contained the highest amount of myricetin (20.79 ± 1.00 μg/mg extract). The antioxidant capacity of hydrolysed extracts was evaluated using in vitro DPPH• (2,2-diphenyl-1-picrylhydrazyl) and ABTS•+ [2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] decolourisation tests. The observed radical scavenging capacities of the extracts showed a relationship with the measured flavonol aglycone content and composition. The acidic treatment resulted in an increased free radical scavenging activity compared to the untreated methanol extract. |
Databáze: | OpenAIRE |
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