Adhesin genes and biofilm formation among pediatric Staphylococcus aureus isolates from implant-associated infections

Autor: Charles G. Minard, Anthony R. Flores, Ruth Ann Luna, Catherine E. Foster, Kristina G. Hulten, Melissa Kok, Linda B. Lamberth, Sheldon L. Kaplan
Rok vydání: 2020
Předmět:
Bacterial Diseases
0301 basic medicine
Staphylococcus
Leukocidin
Pathology and Laboratory Medicine
medicine.disease_cause
Pediatrics
Biochemistry
0302 clinical medicine
Medicine and Health Sciences
030212 general & internal medicine
Child
Skin
Extracellular Matrix Proteins
Multidisciplinary
Bacterial Pathogens
Infectious Diseases
Medical Microbiology
Staphylococcus aureus
Medicine
Pathogens
Pediatric Infections
Research Article
Prosthesis-Related Infections
Science
030106 microbiology
Biology
Microbiology
Bacterial genetics
03 medical and health sciences
DNA-binding proteins
Pulsed-field gel electrophoresis
medicine
Humans
Adhesins
Bacterial
Microbial Pathogens
Gene
Staphylococcal Infection
Bacteria
Soft Tissue Infections
Organisms
Biofilm
Biology and Life Sciences
Proteins
Bacteriology
biochemical phenomena
metabolism
and nutrition
Bacterial adhesin
Genes
Bacterial
Biofilms
MSCRAMM
Bacterial Biofilms
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 6, p e0235115 (2020)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0235115
Popis: BackgroundMicrobial surface component recognizing adhesive matrix molecules (MSCRAMMs) facilitate Staphylococcus aureus adherence to host tissue. We hypothesized that S. aureus isolates from implant-associated infections (IAIs) would differ in MSCRAMM profile and biofilm formation in vitro compared to skin and soft tissue infection (SSTI) isolates.MethodsPediatric patients and their isolates were identified retrospectively. IAI and SSTI isolates were matched (1:4). Pulsed field gel electrophoresis was performed to group isolates as USA300 vs. non-USA300. Whole genome sequencing was performed and raw sequence data were interrogated for presence of MSCRAMMs (clfA, clfB, cna, ebh, efb, fnbpA, fnbpB, isdA, isdB, sdrC, sdrD, sdrE), biofilm-associated (icaA,D,B,C), and Panton-Valentine leukocidin (lukSF-PV) genes, accessory gene regulator group, and multilocus sequence types. In vitro biofilm formation was assessed for 47 IAI and 47 SSTI isolates using a microtiter plate assay. Conditional logistic regression was performed for analysis of matched data (STATA11, College Station, TX).ResultsForty-seven IAI and 188 SSTI isolates were studied. IAI isolates were more often methicillin susceptible S. aureus and non-USA300 vs. SSTI isolates [34 (72%) vs. 79 (42%), p = 0.001 and 38 (81%) vs. 57 (30%) p ConclusionsS. aureus IAI isolates were significantly more likely to be MSSA and non-USA300 than SSTI isolates. Carriage of MSCRAMMs and biofilm formation did not differ significantly between isolates. Evaluation of genetic polymorphisms and gene expression profiles are needed to further delineate the role of adhesins in the pathogenesis of IAIs.
Databáze: OpenAIRE
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