Inhibition of δ-aminolevulinic acid dehydratase by trichloroethylene
| Autor: | Masayuki Yamamoto, Akio Koizumi, Hiroyoshi Fujita, Miho Kumai, Masayuki Ikeda, Tetsuo Sadamoto |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Erythrocytes Metabolite Ethylene Chlorohydrin Urine In Vitro Techniques Toxicology Excretion chemistry.chemical_compound Bone Marrow Internal medicine medicine Animals Trichloroethanes Trichloroacetic Acid Trichloroacetic acid Liver injury biology Chemistry Porphobilinogen synthase Porphobilinogen Synthase Rats Inbred Strains medicine.disease Rats Trichloroethylene Endocrinology Liver Biochemistry Phenobarbital Dehydratase biology.protein 5-Aminolevulinate Synthetase medicine.drug |
| Zdroj: | Toxicology. 30:93-102 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/0300-483x(84)90120-3 |
| Popis: | Male Wistar rats (8 animals/group; 180-200 g) were exposed continuously to trichloroethylene (TRI) for 48 or 240 h or methylchloroform (1,1, 1-trichloroethane: MC for 48 h at 50, 400 and 800 ppm. The inhibition of delta-aminolevulinic acid dehydratase (ALA-D) was examined in liver, blood and bone marrow of naive and phenobarbital pretreated animals exposed to TRI. A clear cut dose-effect relationship between the exposure concentration or duration of exposure and the inhibition of ALA-D activity was seen for rats exposed to TRI. In addition to this finding, significant interaction between TRI exposure and phenobarbital treatment was observed in the inhibition of ALA-D in liver and blood. MC did not produce inhibition. Trichloroacetic acid and trichloroethanol failed to inhibit the ALA-D activity in vitro. It seems that a metabolite(s) of TRI other than the above 2 substances may play a role in the inhibition of ALA-D. The inhibition of ALA-D (38% or 48% of the control in liver or in blood, respectively) observed after the 240 h exposure at 400 ppm to TRI was accompanied by the significant elevation of delta-aminolevulinic acid synthase (186% of the control) in liver and the increase in excretion of delta-aminolevulinic acid in urine (142% of the control). This occurred without an apparent weight loss, liver injury or hematological changes. |
| Databáze: | OpenAIRE |
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