Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions
| Autor: | Felipe Trovalim Jordão, Felipe Baena Garcia, Andreia Moreira dos Santos Carmo, Andressa Moreira Siqueira, Fernanda Nascimento Almeida, Luana Prado Rolim de Oliveira, Daniela Rodrigues Colpas, Adriana Feliciano Alves Duran, Glaucia Raquel Luciano Veiga, Diego Marin Fermino, Aline Diniz Cabral, Fernando Luiz Affonso Fonseca, Edmar Silva Santos, Márcia Aparecida Sperança, Carla Moreira Santana, Beatriz da Costa Aguiar Alves, Ivana Barros de Campos |
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| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
Saliva Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-endpoint-PCR Biology Article Specimen Handling Nasopharynx Virology Internal medicine Epidemiology medicine Humans Viral rna Prospective Studies Diagnostic laboratory Pandemics Protocol (science) SARS-CoV-2 COVID-19 qRT-PCR RNA Viral Mass vaccination Molecular diagnosis Sample collection |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2021.114382 |
| Popis: | Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace viral RNA detection by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) on nasopharynx secretions, which requires healthcare facilities and specialized personnel for sample collection. To develop an alternative protocol, hydrophilic cotton as the material and saliva as the source for biological sample collection in qRT-PCR/RT-endpoint-PCR SARS-CoV-2 diagnostic methods prepared with local consumables were evaluated using 99 archived nasopharynx samples previously diagnosed as positive for SARS-CoV-2 and 111 prospective saliva samples pared with nasopharynx samples from patients attending the local reference ABC Medical School diagnostic laboratory. The kappa agreement coefficient between the SARS-CoV-2 qRT-PCR and RT-endpoint-PCR was k = 0.97 (95% CI 0.92-1.00) and k = 0.90 (95% CI 0.81-0.99), respectively, on SARS-CoV-2-positive archived samples, with the initial qRT-PCR CT under 25. The agreement coefficient of the SARS-CoV-2 alternative saliva diagnostic protocol, when used to test the paired nasopharynx samples, was k = 0.79 (95% CI 0.56-1,00). These data support that the SARS-CoV-2 diagnostic assay based on self-collected saliva on cotton represents an alternative protocol for mass diagnosis and epidemiological studies in low-income regions. |
| Databáze: | OpenAIRE |
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