New cryoprotectant for cryorefractive surgery
| Autor: | Joseph W. Huff, Katherine L. Kratz-Owens, David J. Schanzlin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Glycerol
Male medicine.medical_specialty Cryoprotectant Cell Survival Corneal Stroma medicine.medical_treatment Keratomileusis Freeze injury Cryosurgery Cornea Corneal Transplantation Cryoprotective Agents Corneal edema medicine Animals Dimethyl Sulfoxide Tromethamine Keratophakia Corneal Haze business.industry eye diseases Sensory Systems Surgery Ophthalmology medicine.anatomical_structure Epikeratophakia Female Rabbits sense organs business |
| Zdroj: | Journal of Cataract and Refractive Surgery. 17:608-612 |
| ISSN: | 0886-3350 |
| Popis: | Cryorefractive surgeries, keratomileusis, keratophakia, and epikeratophakia cause destruction of keratocytes, which may result in postoperative corneal haze. We examined the effects of two cryoprotectants on keratocyte survival following freeze injury. We compared the ability of CPTES and the standard cryoprotectant KM-26 to prevent keratocyte death by altering the length of time corneal tissue was exposed to the cryoprotectant. When corneal stroma was immersed in CPTES for five minutes prior to freezing, 66.5% of the keratocytes survived; when tissue was immersed in KM-26 for the same length of time, 27.5% survived (P less than .01). Immersion for one to 30 minutes in CPTES prior to freezing produced keratocyte viabilities that were 40% to 80% of those of fresh, unfrozen tissue; immersion in KM-26 produced keratocyte viabilities of 20% to 60%. We compared the ability of these cryoprotectants to reduce corneal haze following freeze injury using our rabbit model of lamellar keratoplasty. The postoperative data were comparable to those in the cell culture experiments. Based on our findings in rabbit corneas, a cryoprotective medium such as CPTES may promote cell survival and thereby speed recovery from cryorefractive procedures in humans. |
| Databáze: | OpenAIRE |
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