Basal keratinocyte tetrasomy in low-grade squamous intra-epithelial lesions of the cervix is restricted to high and intermediate risk HPV infection but is not type-specific
| Autor: | Shirley A. Southern, M F Evans, C S Herrington, Athina Giannoudis |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Keratinocytes
Cancer Research medicine.medical_specialty Pathology Uterine Cervical Neoplasms Aneuploidy keratinocyte Chromosome Disorders Cervix Uteri medicine.disease_cause Polymerase Chain Reaction Risk Assessment law.invention law cervix medicine Humans chromosome Papillomaviridae human papillomavirus Cervix In Situ Hybridization Polymerase chain reaction Chromosome Aberrations biology Papillomavirus Infections HPV infection Cytogenetics virus diseases Regular Article medicine.disease biology.organism_classification Tumor Virus Infections medicine.anatomical_structure Oncology Tetrasomy Carcinoma Squamous Cell Female Carcinogenesis |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1054/bjoc.1999.0937 |
| Popis: | Human papillomavirus (HPV) infection appears to be an early event in cervical carcinogenesis with additional abnormalities being required for biological transformation. We have analysed 179 low-grade cervical squamous intra-epithelial lesions (SILs) and 15 normal cervices for the presence of HPV using both in situ hybridization and polymerase chain reaction (PCR). PCR was performed with GP5+/GP6+ primers followed by hybridization using probes for low (HPV 6, 11, 40, 42, 43, 44), intermediate (HPV 31, 33, 35, 39, 51, 52, 58, 59, 66 and 68) and high-risk HPVs (HPV 16, 18, 45 and 56). Interphase cytogenetic analysis using pericentromeric probes for chromosomes 1, 3, 4, 6, 10, 11, 17, 18 and X was also performed to identify numerical chromosomal abnormalities. Tetrasomy of all nine chromosomes was identified within basal keratinocytes, was restricted to epithelia infected with high risk (17 of 46) or intermediate risk (23 of 83) HPVs but was not HPV type-specific. Tetrasomy was not identified in any of the epithelia infected with low risk HPVs (n = 62). These numbers include multiple infection. These findings indicate that the induction of tetrasomy is a property restricted to high and intermediate-risk HPV types but that it is not type-specific. The factors governing which lesions will develop this abnormality are as yet unclear. © 2000 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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