Enhancement of a culture-based bacterial detection system (eBDS) for platelet products based on measurement of oxygen consumption
| Autor: | Mary Anne Cortus, Girolamo A. Ortolano, Barry Wenz, Roslyn Yomtovian, Michael R. Jacobs, Stein Holme, Chiyong Chong, Lawrence F. Freundlich, Morven B. McAlister |
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| Rok vydání: | 2005 |
| Předmět: |
Blood Platelets
Time Factors Staphylococcus Immunology chemistry.chemical_element Bacterial growth Oxygen Sensitivity and Specificity Oxygen Consumption Leukocytes Immunology and Allergy Platelet Food science Incubation Bacteriological Techniques biology Bacteria Inoculation Platelet Count Medical screening Hematology Bacterial Infections biology.organism_classification Kinetics chemistry Evaluation Studies as Topic Limiting oxygen concentration |
| Zdroj: | Transfusion. 45(6) |
| ISSN: | 0041-1132 |
| Popis: | Background An enhanced bacterial detection system (Pall eBDS) was developed that distinguishes itself from its predecessor (Pall BDS) by removal of the platelet (PLT)-retaining filter allowing for optimal bacterial transfer, modification of the culture tablet to reduce the confounding effects of respiring PLTs while enhancing bacterial growth, and facilitation of nutrients and gas exchange by agitating the sample pouch during incubation at 35 degrees C. The objective was to evaluate the performance of the new eBDS. Study design and methods Leukoreduced whole blood-derived PLT concentrates (LR-PCs) and LR single-donor PLTs (LR-SDPs) were inoculated with 1 to 15 colony-forming units (CFUs) of bacteria per mL in studies of each of 10 bacterial species associated with fatal transfusion-transmitted bacterial infection. Immediately after inoculation and after 24 hours of storage at 22 degrees C, samples of inoculated LR-PCs were aseptically transferred into the eBDS pouches. Pouches were then incubated for 24 hours at 35 degrees C with agitation and oxygen concentration was then measured. Results Median inoculation levels ranged from 5 to 13 CFUs per mL for each species studied. No significant differences in oxygen concentration were found when comparing LR-PCs with LR-SDPs. When sampling occurred from the PLTs 24 hours after inoculation, all 280 cases (24-33 replicates of each species) were detected as contaminated by the device (100% sensitivity). No false-positives were obtained with 713 uninoculated PLT units. Conclusions The eBDS demonstrated improved detection sensitivity in the range of 1 to 15 CFUs per mL with no observed false-positives compared to the original BDS (detection range 100 to 500 CFUs/mL) with no false-positives. |
| Databáze: | OpenAIRE |
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