Effect of Daily Hemodialysis on Monocytes Apoptosis
| Autor: | Nathan W. Levin, Emanuela Buoncristiani, Valeria Bordoni, Claudio Ronco, Michail Pappas, Monica Bonello, Vincenzo D'Intini, Emilio Andrikos, Umberto Buoncristiani |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment Apoptosis Monocytes Plasma chemistry.chemical_compound Immune system Renal Dialysis Annexin Internal medicine Humans Medicine Propidium iodide Cells Cultured U937 cell Caspase 3 business.industry Monocyte U937 Cells Hematology General Medicine Middle Aged medicine.disease Uremia medicine.anatomical_structure Endocrinology chemistry Nephrology Caspases Immunology Female Hemodialysis business Peritoneal Dialysis |
| Zdroj: | Blood Purification. 23:79-82 |
| ISSN: | 1421-9735 0253-5068 |
| DOI: | 10.1159/000082015 |
| Popis: | Uremia is associated with a state of immune dysfunction with increased susceptibility to infection and malignancy possibly related to dysregulation of immune system cell apoptosis. Peritoneal dialysis can restore plasma apoptosis activity on monocytes compared to intermittent hemodialysis. Whether the continuous modality or diverse clearance mechanisms involved are responsible is unknown. Apoptosis rates correlate with phagocytic function highlighting the benefit of efficient toxin clearance. The plasma of 16 patients on daily hemodialysis (D-HD) was incubated with U937 monocytes and compared to 18 hemodialysis (HD) patients, 5 chronic renal failure (CRF) subjects and 5 healthy volunteers (controls). Apoptosis was evaluated by immunofluorescence microscopy dyes (Hoechst 33342, propidium iodide) and annexin V cytoflowmetry at 96 h. Plasma-induced U937 apoptosis (mean values) was significantly enhanced in D-HD (18.8 ± 4.1), HD (19.67 ± 5.5) and CRF patients (20.8 ± 4.7) compared to controls (9.6 ± 3.6; p < 0.05 for CRF vs. controls, HD vs. controls and D-HD vs. controls). No significant differences were observed between D-HD, HD and CRF sera on apoptosis rate, caspase-3 activity and phagocytic capacity of U937 monocytes. This study demonstrates that the plasma of various HD schedules was unable to reduce monocyte apoptosis induced by uremia. |
| Databáze: | OpenAIRE |
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