The α-tocopherol status and expression of α-tocopherol-related proteins in methionine-choline deficient rats treated with vitamin E
| Autor: | Hiroshi Miyazaki, Kimitaka Takitani, Hiroshi Tamai, Atsushi Yoden, Maki Koh |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Vitamin
medicine.medical_specialty antioxidant Antioxidant medicine.medical_treatment Clinical Biochemistry Medicine (miscellaneous) Liver disorder Lipid peroxidation chemistry.chemical_compound α-tocopherol transfer protein Internal medicine medicine Tocopherol α-tocopherol Nutrition and Dietetics biology business.industry Vitamin E Fatty liver non-alcoholic fatty liver disease Cytochrome P450 medicine.disease Endocrinology Biochemistry chemistry biology.protein Original Article business |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | Non-alcoholic fatty liver disease is the most common liver disorder in developed countries, and its incidence is increasing in all population groups. As an antioxidant, vitamin E is effective in the treatment of non-alcoholic fatty liver disease, although the mechanism is still unclear. Methionine-choline deficient Wistar rats (n = 5) used as an experimental model of non-alcoholic fatty liver disease were fed a vitamin E-enriched diet (500 mg/kg) for 4 weeks. The effects were assessed by measuring lipid peroxidation, α-tocopherol levels, and the expression of α-tocopherol-related proteins in the liver. In vitamin E-treated methionine-choline deficient rats, lipid peroxidation was reduced, but liver histopathological changes were not improved. Hepatic α-tocopherol levels in these rats were significantly elevated compared to normal rats treated with vitamin E. Expression of liver α-tocopherol transfer protein in vitamin E-treated methionine-choline deficient rats was significantly repressed compared to methionine-choline deficient rats. The expression of liver cytochrome P450 4F2 and ATP-binding cassette transporter protein 1, involved in metabolism and transport of α-tocopherol, respectively, was significantly repressed in vitamin E-treated methionine-choline deficient rats. In methionine-choline deficient rats, vitamin E treatment altered the hepatic α-tocopherol-related protein expression, which may affect α-tocopherol status in the liver, leading to reduced lipid peroxidation. |
| Databáze: | OpenAIRE |
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