Particle-Mediated DNA Immunization of Cattle Confers Long-Lasting Immunity against Bovine Herpesvirus-1
| Autor: | Lorne A. Babiuk, Ralph P. Braun, S. van Drunen Littel-van den Hurk, B. I. Loehr |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
Cattle Diseases Biology Antibodies Viral Green fluorescent protein DNA vaccination Gene gun Mice 03 medical and health sciences 0302 clinical medicine Plasmid Immune system Immunity Virology Vaccines DNA Animals Herpesvirus 1 Bovine 030304 developmental biology 0303 health sciences Viral Vaccines Herpesviridae Infections Transfection Biolistics biology.organism_classification Molecular biology Bovine herpesvirus 1 3. Good health Mice Inbred C57BL Immunoglobulin G DNA Viral Cattle 030215 immunology |
| Zdroj: | Virology. 265:46-56 |
| ISSN: | 0042-6822 |
| Popis: | Particle-mediated delivery was used as a method to vaccinate ruminants with a DNA vaccine. The optimal conditions for gene gun-based delivery of gold particles into the epidermal layer of the skin were determined. After delivery of the gold particles, an inflammatory response was observed. This response occurred regardless of the presence of plasmid and therefore was a result of the physical disturbance of the skin by the gold particles. To identify transfected cells, a plasmid expressing a green fluorescent protein was delivered into the skin. Fluorescent cells were located primarily in the outermost layers of the epidermis and outside the core of gold particles deposited by the gene gun. Cattle were immunized by gene gun with a plasmid expressing a truncated, secreted form of bovine herpesvirus-1 glycoprotein D. Serum antibody responses, antigen-specific proliferation, and interferon-γ secretion by peripheral blood lymphocytes were demonstrated. These immune responses were found to be of long duration and sufficient magnitude to protect cattle against challenge with bovine herpesvirus-1, which demonstrates the efficacy of gene gun-based delivery of DNA vaccines to target species. |
| Databáze: | OpenAIRE |
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