Specific induction of a 72-kDa heat shock protein protects esophageal mucosa from reflux esophagitis
| Autor: | Sumio Watanabe, Michiro Otaka, Makiko Takada, Yuji Shimada, Daisuke Asaoka, Yuko Izumi, Akihito Nagahara, Taiji Takahashi, Hideaki Itoh |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Hyperthermia medicine.medical_specialty Blotting Western HSP72 Heat-Shock Proteins Gastroenterology General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine Rats Sprague-Dawley Western blot Internal medicine Heat shock protein medicine Animals General Pharmacology Toxicology and Pharmaceutics Reflux esophagitis Esophagus Esophagitis Peptic Mucous Membrane biology medicine.diagnostic_test business.industry Hyperthermia Induced General Medicine medicine.disease Hsp90 Rats Disease Models Animal medicine.anatomical_structure biology.protein HSP60 business |
| Zdroj: | Life Sciences. 84:517-522 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2009.01.016 |
| Popis: | Aims The aim of this study is to investigate the expression and cytoprotective function of a 72-kDa heat shock protein (HSP72) using a reflux esophagitis model in rats. Main methods Expression of HSP60, HSP72, and HSP90 in rat esophageal mucosa was evaluated by Western blot analysis before and after hyperthermia (42.5 °C, 20 min). Rats received the operation to produce reflux esophagitis with or without pretreatment with hyperthermia to induce HSPs. The esophageal mucosal damage was evaluated 12 h after the operation. Key findings Expression of HSP72 was significantly increased by hyperthermia in rat esophageal mucosa. Reflux esophagitis was dramatically prevented when HSP72 was preinduced by hyperthermia. Furthermore, activation of TNF-α and IL-1β in esophageal mucosa was also suppressed. Significance These results suggested that hyperthermia protects the esophageal mucosa in reflux esophagitis model by inducing HSP72 and suppressing proinflammatory cytokine activation. These findings might suggest that HSP-inducing therapy could be a novel and unique therapy for reflux esophagitis. |
| Databáze: | OpenAIRE |
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