Consecutive CT in vivo lung imaging as quantitative parameter of influenza vaccine efficacy in the ferret model
| Autor: | Jorma Hinkula, Koert J. Stittelaar, Leon de Waal, Edwin J. B. Veldhuis Kroeze, Gabriel P. Krestin, Geert van Amerongen, Marcel L. Dijkshoorn, Vera J. P. Teeuwsen, Thijs Kuiken, Albert D. M. E. Osterhaus |
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| Přispěvatelé: | Virology, Radiology & Nuclear Medicine, Internal Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
CT scan
Medicin och hälsovetenskap Influenza vaccine Medical and Health Sciences Virus Serum antibody Placebos SDG 3 - Good Health and Well-being Orthomyxoviridae Infections In vivo Immunology and Microbiology(all) Lung imaging Drug Discovery Pathology Ferret Medicine Animals Lung Preclinical vaccine efficacy General Veterinary General Immunology and Microbiology business.industry Public Health Environmental and Occupational Health Ferrets Vaccine efficacy veterinary(all) Virology Influenza Titer Disease Models Animal Infectious Diseases Treatment Outcome Influenza Vaccines In vivo imaging Immunology Molecular Medicine Female business Tomography X-Ray Computed influenza Preclinical imaging |
| Zdroj: | Vaccine, 30(51), 7391-7394. Elsevier |
| ISSN: | 0264-410X |
| Popis: | Preclinical vaccine efficacy studies are generally limited to certain read out parameters such as assessment of virus titers in swabs and organs, clinical signs, serum antibody titers, and pathological changes. These parameters are not always routinely applied and not always scheduled in a logical standardized way. We used computed tomography (CT) imaging as additional and novel read out parameter in a vaccine efficacy study by quantifying alterations in aerated lung volumes in ferrets challenged with the 2009 pandemic A/H1N1 influenza virus. Vaccination protected from marked variations in aerated lung volumes compared to naive controls. The vaccinated group showed a daily gradual mean reduction with a maximum of 7.8%, whereas the controls showed a maximum of 143% reduction. The pulmonary opacities evident on CT images were most pronounced in the placebo-treated controls, and corresponded to significantly increased relative lung weights at necropsy. This study shows that consecutive in vivo CT imaging allows for a day to day read out of vaccine efficacy by quantification of altered aerated lung volumes. (C) 2012 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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