Activation of rabbit blood platelets by anandamide through its cleavage into arachidonic acid
| Autor: | Lhousseine Touqui, Sandrine Braud, Cassian Bon, Carine Mounier |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
Cannabinoid receptor Biophysics Cannabinoid ligand Pharmacology Biochemistry chemistry.chemical_compound immune system diseases Structural Biology Genetics Amidase activity Platelet Platelet activation skin and connective tissue diseases Molecular Biology fungi Cell Biology Anandamide stomatognathic diseases chemistry Cannabinoid receptor antagonist Arachidonic acid PMSF |
| Zdroj: | FEBS Letters. 471:12-16 |
| ISSN: | 0014-5793 |
| Popis: | Anandamide (ANA), a cannabinoid receptor ligand, stimulated platelet aggregation at concentrations similar to those of arachidonic acid (AA). The aggregating effect of ANA was inhibited by aspirin but not by SR-141716, a cannabinoid receptor antagonist. In addition, HU-210, a cannabinoid receptor agonist, failed to induce platelet activation. Radiolabelling experiments showed that exogenous ANA was cleaved by platelets into AA through a phenylmethylsulfonyl fluoride (PMSF)-sensitive pathway. In agreement, PMSF was shown to abolish the aggregating effect of ANA. In conclusion, ANA is able to induce platelet activation via its cleavage by a PMSF-sensitive amidase activity, leading to the release of AA which in turn activates platelets. |
| Databáze: | OpenAIRE |
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