Design of a nanocomposite substrate inducing adult stem cell assembly and progression toward an Epiblast-like or Primitive Endoderm-like phenotype via mechanotransduction

Autor: Pia Montanucci, Chiara Argentati, Ilaria Pennoni, Giuseppe Basta, Teresa Pescara, Francesco Morena, Simona Montesano, Elena Fortunati, Sabata Martino, Luigi Torre, Samantha Mattioli, Riccardo Calafiore, Carla Emiliani, I. Armentano, Jose Maria Kenny, Ilaria Bicchi, Loredana Latterini
Rok vydání: 2017
Předmět:
Adult
0301 basic medicine
Homeobox protein NANOG
Materials science
Polyesters
Biophysics
Biocompatible Materials
Bioengineering
Nanotechnology
02 engineering and technology
Stem cell reprogramming
Mechanotransduction
Cellular
Nanocomposites
Biomaterials
Adherens junction
Focal adhesion
03 medical and health sciences
NANOG and GATA6 expression
Tissue engineering
Humans
Cellular Reprogramming Techniques
Mechanotransduction
Cells
Cultured
Tissue Engineering
Nanotubes
Carbon
Endoderm
biochemical mechanotransduction
Stem cell-biomaterial interface
Cellular Reprogramming
Fetal Blood
021001 nanoscience & nanotechnology
Adult Stem Cells
030104 developmental biology
Mechanics of Materials
embryonic structures
Ceramics and Composites
Stem cell
0210 nano-technology
Reprogramming
Germ Layers
Adult stem cell
Zdroj: Biomaterials. 144:211-229
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2017.08.015
Popis: This work shows that the active interaction between human umbilical cord matrix stem cells and Poly ( l -lactide)acid (PLLA) and PLLA/Multi Walled Carbon Nanotubes (MWCNTs) nanocomposite films results in the stem cell assembly as a spheroid conformation and affects the stem cell fate transition. We demonstrated that spheroids directly respond to a tunable surface and the bulk properties (electric, dielectric and thermal) of plain and nanocomposite PLLA films by triggering a mechanotransduction axis. This stepwise process starts from tethering of the cells' focal adhesion proteins to the surface, together with the adherens junctions between cells. Both complexes transmit traction forces to F-Actin stress fibres that link Filamin-A and Myosin-IIA proteins, generating a biological scaffold, with increased stiffening conformation from PLLA to PLLA/MWCNTs, and enable the nucleoskeleton proteins to boost chromatin reprogramming processes. Herein, the opposite expression of NANOG and GATA6 transcription factors, together with other lineage specification related proteins, steer spheroids toward an Epiblast-like or Primitive Endoderm-like lineage commitment, depending on the absence or presence of 1 wt% MWCNTs, respectively. This work represents a pioneering effort to create a stem cell/material interface that can model the stem cell fate transition under growth culture conditions.
Databáze: OpenAIRE
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