Early inhibition of nitric oxide production increases HSV-1 intranasal infection
| Autor: | Maria C. Courreges, Gisela Gamba, Fabián Benencia, Hernan Cavalieri, Ernesto J. Massouh |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Ratón Immunoblotting Pneumonia Viral Dot blot Nitric Oxide Synthase Type II Inflammation Herpesvirus 1 Human Biology medicine.disease_cause Nitric Oxide Turbinates Virus Replication Guanidines Virus Nitric oxide chemistry.chemical_compound Mice Virology medicine Animals RNA Messenger Lung Pneumonitis Mice Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction Respiratory infection Brain Herpes Simplex Pneumonia medicine.disease Survival Analysis Disease Models Animal Infectious Diseases Herpes simplex virus chemistry Gene Expression Regulation medicine.symptom Nitric Oxide Synthase |
| Zdroj: | Journal of medical virology. 73(2) |
| ISSN: | 0146-6615 |
| Popis: | Here, we studied the role of nitric oxide (NO) production during the first steps of the respiratory infection of BALB/c mice with herpes simplex virus type 1 (HSV-1), strain F. Nitric oxide synthase II (NOS-II) mRNA and protein were detected by reverse transcription (RT)-PCR and dot blot, respectively in samples of lungs and turbinates early post-infection (p.i.). Immunohistochemical analysis revealed pulmonar macrophages and PMN expressing NOS-II in the lungs of infected animals. Animals intranasally treated with aminoguanidine (AG), a NOS inhibitor, during the first steps of infection, showed a dose-dependent increase in pneumonitis compared to controls. Viral titres in turbinates, lungs, and brains were higher in AG treated mice. Finally, histopathology studies revealed a stronger inflammation in eyes, and lungs of these animals. Taken together, these results suggest a role of NO in controlling primary HSV intranasal infection. |
| Databáze: | OpenAIRE |
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