ON THE CONTENT OF CYTOCHROME C IN HUMAN MUSCLES
| Autor: | Rosemarie Simon, Åke Åkeson, Gunnar Biörck |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Heart Ventricles Sodium Diaphragm chemistry.chemical_element Cardiomegaly chemistry.chemical_compound Methods Internal Medicine medicine Humans Ammonium Centrifugation Heme Aged Chromatography biology business.industry Muscles Cytochrome c Skeletal muscle Anemia Heart Metabolism Middle Aged medicine.anatomical_structure chemistry Biochemistry Myoglobin Spectrophotometry biology.protein Cytochromes Female business |
| Zdroj: | Acta Medica Scandinavica. 185:287-292 |
| ISSN: | 0001-6101 |
| DOI: | 10.1111/j.0954-6820.1969.tb07335.x |
| Popis: | A quantitative method of determining cytocrome c in human muscles is described. A piece of muscle, about 4 g, is ground with dry ice, homogenized and exhaustively extracted with phosphate buffer of pH 7.4 containing 0.4 M sodium chloride. After being centrifuged at 15,000 × g the supernatant is acidified to pH 4.0 at which pH cytochrome c remains in solution, and a large coloured precipitate is removed by centrifugation. The supernatant is neutralized and all remaining heme groups containing proteins except cytochrome c are precipitated with ammonium sulphate at 60% saturation. The cytochrome c is then determined by spectrophotometry and calculated as mg per 100 g of dry weight, the latter being determined on the original homogenate. In an autopsy material, mainly consisting of elderly patients, the cytochrome c content was found to be about 105 mg per 100 g dry muscle in the left ventricular myocardium, 90 mg in the right ventricular myocardium, 33 mg in the diaphragm and 18 mg in upper thigh muscles. The relation between heart and skeletal muscle was of the order of 6:1. The limited number of subjects studied does not permit any definite conclusions as regards the relationship of the cytochrome c content to other biological or clinical parameters in the material. The values obtained are higher than reported previously (about twice or more), whereas the relations between values for heart, diaphragm and other skeletal muscle seem to be quite similar to those given earlier. Some comments are given to explain the differences. It is thought that this method, as well as that for myoglobin recently reported, may be of value for the study of the physiology and pathology of iron metabolism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|