Radiolabeled mannosylated dextran derivatives bearing an NIR-fluorophore for sentinel lymph node imaging
| Autor: | Catarina Xavier, Vicky Caveliers, Johannes Heemskerk, Sophie Hernot, Isabel Santos, Maurício Morais, João D. G. Correia, Maria Paula Cabral Campello, Tony Lahoutte |
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| Přispěvatelé: | Supporting clinical sciences, Medical Imaging, Translational Imaging Research Alliance |
| Rok vydání: | 2014 |
| Předmět: |
Biodistribution
Fluorophore Infrared Rays Sentinel lymph node Biomedical Engineering Pharmaceutical Science Bioengineering Gallium Radioisotopes chemistry.chemical_compound Intraoperative Period Nuclear magnetic resonance medicine DOTA Animals Rats Wistar Radionuclide Imaging Pharmacology medicine.diagnostic_test business.industry Organic Chemistry Optical Imaging Dextrans Rats Radiography Dextran Lymphatic system chemistry Positron emission tomography Isotope Labeling Female Lymph Nodes Nuclear medicine business Mannose Emission computed tomography Biotechnology |
| Zdroj: | Bioconjugate chemistry. 25(11) |
| ISSN: | 1520-4812 |
| Popis: | Current methods for sentinel lymph node (SLN) mapping involve the use of radioactivity detection with technetium-99m sulfur colloid and/or visually guided identification using a blue dye. To overcome the kinetic variations of two individual imaging agents through the lymphatic system, we report herein on two multifunctional macromolecules, 5a and 6a, that contain a radionuclide ( 99m Tc or 68 Ga) and a near-infrared (NIR) reporter for pre- and/or intraoperative SLN mapping by nuclear and NIR optical imaging techniques. Both bimodal probes are dextran-based polymers (10 kDa) functionalized with pyrazole-diamine (Pz) or 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetra- acetic acid (DOTA) chelating units for labeling with fac-( 99m Tc(CO)3) + or 68 Ga(III), respectively, mannose units for receptor targeting, and NIR fluorophore units for optical imaging. The probes allowed a clear visualization of the popliteal node by single-photon emission computed tomography (SPECT/CT) or positron emission tomography (PET/CT), as well as real-time optically guided excision. Biodistribution studies confirmed that both macromolecules present a significant accumulation in the popliteal node (5a: 3.87 ± 0.63% IA/organ; 6a: 1.04 ± 0.26% IA/ organ), with minimal spread to other organs. The multifunctional nanoplatforms display a popliteal extraction efficiency >90%, highlighting their potential to be further explored as dual imaging agents. |
| Databáze: | OpenAIRE |
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