Nine introns with conserved boundary sequences in the Euglena gracilis chloroplast ribulose-1,5-bisphosphate carboxylase gene
| Autor: | Jeffrey C. Gingrich, Gary L. Stiegler, Barbara Koller, Hajo Delius, Richard B. Hallick, Michael A. Farley |
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| Rok vydání: | 1984 |
| Předmět: |
Chloroplasts
Euglena gracilis Macromolecular Substances Sequence analysis Ribulose-Bisphosphate Carboxylase ved/biology.organism_classification_rank.species Euglena General Biochemistry Genetics and Molecular Biology Conserved sequence Species Specificity Consensus sequence RNA Messenger Gene Genetics Base Sequence biology ved/biology Intron DNA Restriction Enzymes Plants biology.organism_classification Molecular biology Restriction enzyme Genes Plasmids |
| Zdroj: | Cell. 36:545-553 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(84)90247-2 |
| Popis: | The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spinacea oleracea, by electron microscopy of Euglena rbcL DNA-mRNA hybrids, and by cloning, restriction endonuclease analysis, and partial DNA sequencing. The identification, locus, and coding properties for six of ten exons was confirmed by partial DNA sequence analysis. Each of the nine introns in the approximately 6.5 kb rbcL locus is approximately 0.5 kb in length. The DNA sequence of five 3'-intron/5'-exon and four 3'-exon/5'-intron boundaries are highly conserved. A proposed consensus sequence is (formula; see text) These conserved sequences could play a role in an mRNA splicing mechanism in chloroplasts analogous to that in eucaryotic nuclei. |
| Databáze: | OpenAIRE |
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