Short- and long-term gene expression profiles induced by inhaled TiO2 nanostructured aerosol in rat lung
| Autor: | Stéphane Grossmann, Doulaye Dembélé, Stéphane Binet, Hélène Dubois-Pot-Schneider, Frédéric Cosnier, Sylvie Michaux, Sylvie Sébillaud, Carole Seidel, Olivier Joubert, Bertrand H. Rihn, Mylène Lorcin, Cristina Langlais, Laëtitia Chézeau, Hervé Nunge, Ramia Safar, Laurent Gaté |
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| Přispěvatelé: | Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), Cibles thérapeutiques, formulation et expertise pré-clinique du médicament (CITHEFOR), Université de Lorraine (UL), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lung inflammation [SDV]Life Sciences [q-bio] Inflammation Pharmacology Toxicology medicine.disease_cause Short- and long- term expression 03 medical and health sciences [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Gene expression medicine Aerosolization Lung Inhalation medicine.diagnostic_test Chemistry Gene expression profile 3. Good health Gene expression profiling 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure [SDV.TOX]Life Sciences [q-bio]/Toxicology Titanium dioxide medicine.symptom Oxidative stress |
| Zdroj: | Toxicology and Applied Pharmacology Toxicology and Applied Pharmacology, Elsevier, 2018, 356, pp.54-64. ⟨10.1016/j.taap.2018.07.013⟩ |
| ISSN: | 0041-008X 1096-0333 |
| Popis: | International audience; The number of workers potentially exposed to nanoparticles (NPs) during industrial processes is increasing, although the toxicological properties of these compounds still need to be fully characterized. As NPs may be aerosolized during industrial processes, inhalation represents their main route of occupational exposure. Here, the short- and long-term pulmonary toxicological properties of titanium dioxide were studied, using conventional and molecular toxicological approaches. Fischer 344 rats were exposed to 10 mg/m3 of a TiO2 nanostructured aerosol (NSA) by nose-only inhalation for 6 h/day, 5 days/week for 4 weeks. Lung samples were collected up to 180 post-exposure days. Biochemical and cytological analyses of bronchoalveolar lavage (BAL) showed a strong inflammatory response up to 3 post-exposure days, which decreased overtime. In addition, gene expression profiling revealed overexpression of genes involved in inflammation that was maintained 6 months after the end of exposure (long-term response). Genes involved in oxidative stress and vascular changes were also up-regulated. Long-term response was characterized by persistent altered expression of a number of genes up to 180 post-exposure days, despite the absence of significant histopathological changes. The physiopathological consequences of these changes are not fully understood, but they should raise concerns about the long-term pulmonary effects of inhaled biopersistent NPs such as TiO2. |
| Databáze: | OpenAIRE |
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