Effect of oxygen limitation on the content of n-hexadecane-inducible cytochrome P-450 in Acinetobacter calcoaceticus strain EB 104
| Autor: | Sharychev Aa, Matyashova Rn, O. Asperger, Hans-Peter Kleber, Losinov Ab |
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| Rok vydání: | 1986 |
| Předmět: |
Cytochrome
biology Acinetobacter Cytochrome d Cytochrome P450 chemistry.chemical_element General Medicine Metabolism biology.organism_classification Applied Microbiology and Biotechnology Oxygen Oxygen tension Kinetics Biochemistry chemistry Cytochrome P-450 Enzyme System Enzyme Induction Respiration Alkanes biology.protein Acinetobacter calcoaceticus Anaerobiosis |
| Zdroj: | Journal of basic microbiology. 26(10) |
| ISSN: | 0233-111X |
| Popis: | The content of cytochrome P-450 as a function of oxygen supply was studied during growth of Acinetobacter on n-hexadecane in batch cultures at constant pH and agitation. The rate of growth and the content of cytochrome P-450 were not affected as long as the dissolved oxygen tension ranged above 3 to 5% of saturation. The amount of cytochrome P-450 increased when the oxygen tension declined to zero. Cytochrome P-450 levels of about 0.3 to 0.4 nmol/mg protein, i.e. a more than a threefold increase, were observed under conditions where oxygen supply was strictly limited and allowed to maintain only a minimum of metabolism or growth. Limited oxygen supply exerted a special effect on the induction of the cytochrome P-450 as concluded from an increasing ratio between cytochrome P-450 and cytochrome o, and from the absence of cytochrome d in cells with elevated content of cytochrome P-450. The increased formation of cytochrome P-450 was a reversible process. |
| Databáze: | OpenAIRE |
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