Human parainfluenza virus type 4 infections: a report of 20 cases from 1998 to 2002
| Autor: | Astrid Vabret, Geneviève Billaud, Michèle Aymard, Nicolas Crassard, Claire Galambrun, François Freymuth, Florence Morfin, Anne Boucher, Danielle Thouvenot, Bruno Lina, Loïc Legrand, Olivier Ferraris, Yves Gillet |
|---|---|
| Přispěvatelé: | Rollin, Bertrand, Virologie et pathogenèse virale (VPV), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Virologie Humaine et Moléculaire [Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Service de Pediatrie Debrousse, Hôpital Debrousse, Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Adult
Paramyxoviridae Respiratory System Specimen Handling/methods Human/genetics/isolation & purification Virus Specimen Handling Cell Line Rubulavirus Infections Nasopharynx Virology Humans Nasopharynx/virology Paramyxovirinae Rubulavirus OCIS 000.1430 Mononegavirales Child Cytopathic effect DNA Primers [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction biology.organism_classification Parainfluenza Virus 4 Human Human Parainfluenza Virus Rubulavirus Infections/*epidemiology Infectious Diseases Respiratory System/virology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Parainfluenza Virus 4 Pharynx Pharynx/virology |
| Zdroj: | J Clin Virol J Clin Virol, 2005, pp.51 |
| Popis: | BACKGROUND: Human Parainfluenza Viruses (HPIV) type 4 are responsible for respiratory infections. Unlike HPIV types 1-3, they are associated with mild infections and appear to be infrequent. Thus, they often go undetected. STUDY DESIGN: From 1998 to 2002, in 20 respiratory samples of hospitalised patient, we isolated viruses presenting a large syncytial cytopathic effect when inoculated on LLC-MK2 cells. Most of the patients (16/20) were young infants and all of them presented with respiratory infections. RESULTS: We detected 18 cases during autumn and winter, 1 case during spring and 1 during summer. We could not identify these viruses using the panel of routine assays. Samples were then analysed by specific HPIV 4 RT-PCR and IF assays. All the samples were scored positive with both methods. CONCLUSION: We conclude that HPIV 4 infections are probably underestimated. Their role in viral respiratory infections should be carefully investigated using techniques adapted to their detection and culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|