Bridging-induced phase separation induced by cohesin SMC protein complexes
| Autor: | Allard J. Katan, Davide Marenduzzo, Frank Uhlmann, Céline Bouchoux, Davide Michieletto, Ralph de Groot, Je-Kyung Ryu, Andrea Bonato, Eugene Kim, Hon Wing Liu, Masashi Minamino, Cees Dekker |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chromosomal Proteins
Non-Histone Biophysics Cell Cycle Proteins Saccharomyces cerevisiae Chromosomes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Research Articles 030304 developmental biology Genomic organization 0303 health sciences Multidisciplinary Cohesin SMC protein SciAdv r-articles Life Sciences Chromosome Fluorescence recovery after photobleaching DNA eye diseases Yeast 3. Good health Chromatin chemistry biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery Research Article |
| Zdroj: | Ryu, J-K, Bouchoux, C, Liu, H W, Kim, E, Minamino, M, de Groot, R, Katan, A J, Bonato, A, Marenduzzo, D, Michieletto, D, Uhlmann, F & Dekker, C 2021, ' Bridging-induced phase separation induced by cohesin SMC protein complexes ', Science Advances, vol. 7, no. 7, eabe5905, pp. 1-10 . https://doi.org/10.1126/sciadv.abe5905 Science Advances 7 Science Advances Science Advances, 7(7) |
| ISSN: | 2375-2548 |
| Popis: | Yeast cohesin complexes cluster onto long DNA into liquid droplets, which they accomplish by ATP-independent DNA bridging. Structural maintenance of chromosome (SMC) protein complexes are able to extrude DNA loops. While loop extrusion constitutes a fundamental building block of chromosomes, other factors may be equally important. Here, we show that yeast cohesin exhibits pronounced clustering on DNA, with all the hallmarks of biomolecular condensation. DNA-cohesin clusters exhibit liquid-like behavior, showing fusion of clusters, rapid fluorescence recovery after photobleaching and exchange of cohesin with the environment. Strikingly, the in vitro clustering is DNA length dependent, as cohesin forms clusters only on DNA exceeding 3 kilo–base pairs. We discuss how bridging-induced phase separation, a previously unobserved type of biological condensation, can explain the DNA-cohesin clustering through DNA-cohesin-DNA bridges. We confirm that, in yeast cells in vivo, a fraction of cohesin associates with chromatin in a manner consistent with bridging-induced phase separation. Biomolecular condensation by SMC proteins constitutes a new basic principle by which SMC complexes direct genome organization. |
| Databáze: | OpenAIRE |
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