Lifetimes of Arabidopsis cryptochrome signaling states in vivo
| Autor: | Robert Bittl, Ringo Wenzel, Vera Herbel, Christian Orth, Alfred Batschauer, Margaret Ahmad |
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| Přispěvatelé: | Photobiologie (PHOTO), Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Deutsche Forschungsgemeinschaft (DFG) [BA985/12-1, Bi464/8-3], [HFSP-RGP0045/2008-C], [NSF 33701], Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Photoreceptors Plant signaling state Insecta Light Signal Transduction Time Factors Semiquinone Light Arabidopsis thaliana [SDV]Life Sciences [q-bio] Arabidopsis Plant Science 01 natural sciences law.invention Cell Line 03 medical and health sciences Cryptochrome In vivo law Gene Expression Regulation Plant Genes Reporter Botany Genetics Animals Electron paramagnetic resonance 030304 developmental biology 0303 health sciences biology photoreceptor lifetime Arabidopsis Proteins fungi Cell Biology Chromophore biology.organism_classification Plants Genetically Modified Cryptochromes electron paramagnetic resonance Seedlings Mutation Biophysics Flavin-Adenine Dinucleotide sense organs cryptochrome Ground state 010606 plant biology & botany flavin radical |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2013, 74 (4), pp.583-592. ⟨10.1111/tpj.12144⟩ Plant Journal, 2013, 74 (4), pp.583-592. ⟨10.1111/tpj.12144⟩ |
| ISSN: | 0960-7412 1365-313X |
| Popis: | International audience; One crucial component in light signaling is the quantity of photoreceptor present in the active signaling state. The lifetime of the signaling state of a photoreceptor is limited because of thermal or otherwise back reversion of the chromophore to the ground state, and/or degradation of the photoreceptor in the light-activated state. It was previously shown that the lit state of plant cryptochromes contains flavin-neutral semiquinone, and that the half-lives of the lit state were in the range of 34min in vitro. However, it was unknown how long-lived the signaling states of plant cryptochromes are in situ. Based on the loss of degradation of cry2 after prolonged dark incubation and loss of reversibility of photoactivated cry1 by a pulse of green light, we estimate the in vivo half-lives of the signaling states of cry1 and cry2 to be in the range of 5 and 16min, respectively. Based on electron paramagnetic resonance measurements, the lifetime of the Arabidopsis cry1 lit state in insect cells was found to be similar to 6min, and thus very similar to the lifetime of the signaling state in planta. Thus, the signaling state lifetimes of plant cryptochromes are not, or are only moderately, stabilized in planta. |
| Databáze: | OpenAIRE |
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