Suppression of interleukin 1alpha and interleukin 1beta in human limbal epithelial cells cultured on the amniotic membrane stromal matrix
| Autor: | Dagoberto Monroy, Zhonghua Ji, Scheffer C.G. Tseng, Abraham Solomon, Mark I. Rosenblatt, Stephen C. Pflugfelder |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Stromal cell Lipopolysaccharide medicine.medical_treatment Gene Expression Enzyme-Linked Immunosorbent Assay Inflammation Limbus Corneae Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Ribonucleases medicine Humans Amnion Cells Cultured Biological Dressings Epithelium Corneal Glyceraldehyde-3-Phosphate Dehydrogenases Receptors Interleukin-1 Interleukin Molecular biology Sensory Systems Epithelium Up-Regulation Transplantation Ophthalmology medicine.anatomical_structure Cytokine chemistry Cell culture Original articles - Laboratory science Immunology Female medicine.symptom Interleukin-1 |
| Zdroj: | British Journal of Ophthalmology. 85:444-449 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.85.4.444 |
| Popis: | AIMS—Amniotic membrane (AM) transplantation reduces inflammation in a variety of ocular surface disorders. The aim of this study was to determine if AM stroma suppresses the expression of the IL-1 gene family in cultured human corneal limbal epithelial cells. METHODS—Human corneal limbal epithelial cells were cultured from limbocorneal explants of donor eyes on plastic or on the AM stroma. Transcript expression of IL-1α, IL-1β, IL-1 receptor antagonist (RA), and GAPDH was compared with or without addition of lipopolysaccharide to their serum-free media for 24 hours using RNAse protection assay (RPA). Their protein production in the supernatant was analysed by ELISA. RESULTS—Expression of IL-1α and IL-1β transcripts and proteins was significantly reduced by cells cultured on the AM stromal matrix compared with plastic cultures whether lipopolysaccharide was added or not. Moreover, expression of IL-1 RA by cells cultured in the lipopolysaccharide-free medium was upregulated by AM stromal matrix. The ratio between IL-1 RA and IL-1α protein levels in AM cultures was higher than in plastic cultures. CONCLUSIONS—AM stromal matrix markedly suppresses lipopolysaccharide induced upregulation of both IL-1α and IL-1β. These data may explain in part the effect of AM transplantation in reducing ocular surface inflammation, underscoring the unique feature of the AM as a substrate for tissue engineering. |
| Databáze: | OpenAIRE |
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