Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes
| Autor: | Alexandrina Ferreira Mendes, M. Ribeiro, Fernando Judas, Ana T. Rufino, Cátia Sousa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology medicine.drug_class Clinical Biochemistry Estrogen receptor Biology Chondrocyte Receptors G-Protein-Coupled 03 medical and health sciences Chondrocytes 0302 clinical medicine medicine Humans Receptor Mitogen-Activated Protein Kinase 3 Cartilage homeostasis Cartilage Estrogen Receptor alpha Estrogens Cell Biology Cell biology 030104 developmental biology medicine.anatomical_structure Receptors Estrogen Estrogen 030220 oncology & carcinogenesis Signal transduction GPER Signal Transduction |
| Zdroj: | Journal of Cellular Physiology. 235:8486-8494 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.29691 |
| Popis: | Estrogen hormones are important for cartilage homeostasis, but nothing is known regarding the expression and role of the membrane G protein-coupled estrogen receptor (GPER), G protein-coupled receptor 30 (GPR30), in adult articular chondrocytes. Using immunohistochemistry of cartilage sections, quantitative real-time polymerase chain reaction and Western blot of chondrocyte extracts, we found that these cells express GPR30. Nonetheless, the pattern of bands detected by two distinct antibodies does not overlap, suggesting that the proteins detected represent partially degraded forms of the receptor. Treatment with GPR30 agonists did not induce Akt or ERK1/2 phosphorylation, two known GPR30-activated signaling pathways, suggesting that GPR30 is not functional in human chondrocytes. Therefore, the protective anti-osteoarthritic role of estrogen hormones in cartilage homeostasis is likely independent of GPR30. This study was performed using human cartilage collected from the distal femoral condyles of multiorgan donors at the Bone and Tissue Bank of the University and Hospital Center of Coimbra. |
| Databáze: | OpenAIRE |
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