Carrageenan stimulates reduction of nitroblue tetrazolium by human neutrophils without membrane depolarization, myeloperoxidase secretion, or increased oxygen consumption
| Autor: | Eric P. Brestel, Vincent Castranova, Edward J. McClain |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Neutrophils
Immunology Tetrazolium Salts Inflammation Pharmacology Granulocyte In Vitro Techniques Carrageenan Membrane Potentials chemistry.chemical_compound Oxygen Consumption medicine Immunology and Allergy Humans Peroxidase biology Superoxide Nitroblue Tetrazolium Zymosan Cell Membrane Depolarization Hydrogen Peroxide carbohydrates (lipids) Kinetics medicine.anatomical_structure chemistry Biochemistry Myeloperoxidase biology.protein Tetradecanoylphorbol Acetate medicine.symptom Oxidation-Reduction Histamine |
| Zdroj: | Inflammation. 10(4) |
| ISSN: | 0360-3997 |
| Popis: | Carrageenan, a sulfated polyanionic polysaccharide, is commonly used to induce inflammation in experimental animals, and this model is used to screen for the effectiveness of antiinflammatory drugs. Carrageenan-induced inflammation has been attributed to a variety of autocoids including histamine, bradykinin, complement, Superoxide, and prostaglandins. This study examines the effects of carrageenan on human PMN in a serum-free system. Carrageenan was found to stimulate the reduction of NBT by PMNs without stimulating membrane depolarization, oxygen consumption, H2O2 production, or myeloperoxidase secretion. Carrageenan stimulates a heat-labile, NBT-reducing system which is unassociated with the usual stimulus-response coupling seen with other PMN activators such as PMA, FMLP, and zymosan. |
| Databáze: | OpenAIRE |
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