Expression of cytokeratins (CKs) 8, 13 and 18 and their mRNA in epithelial linings of radicular cysts: implication for the same CK profiles as nasal columnar epithelium in squamous epithelial lining
| Autor: | D P Lu, T Kimura, T Osaki, Y Tatemoto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty In situ hybridization Biology Immunoenzyme Techniques Cytokeratin medicine Humans Cyst RNA Messenger General Dentistry In Situ Hybridization Radicular Cyst Reverse Transcriptase Polymerase Chain Reaction Epithelial Cells RNA Probes Anatomy Middle Aged medicine.disease Epithelium Staining Reverse transcription polymerase chain reaction Nasal Mucosa stomatognathic diseases Phenotype medicine.anatomical_structure Otorhinolaryngology Keratins Immunohistochemistry Female |
| Zdroj: | Oral Diseases. 8:30-36 |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1034/j.1601-0825.2002.1o786.x |
| Popis: | OBJECTIVE: Our objective was to specify cytokeratin (CK) patterns in lining epithelia of radicular cysts which are sometime lined with ciliated columnar epithelia as seen in the nasal epithelia. MATERIALS AND METHODS: We examined the CK expression in 52 radicular cysts obtained from 32 maxillary and 20 mandibular lesions and investigated CK-mRNA expression using in situ hybridization in 24 maxillary and 13 mandibular cysts and reverse transcription-polymerase chain reaction (RT–PCR) in 24 maxillary cysts. RESULTS: Of the maxillary cysts, 20, 29 and 19 squamous epithelial linings were positive for CK8, CK13 and CK18, respectively; of the mandibular cysts, 10, 20 and 11 linings were positive for these CKs, respectively. The expression patterns of CK18(+)-CK13(−), CK18(+)-CK13(+) and CK18(−)-CK13(+) were observed in 3, 16 and 13 linings of the maxillary cysts and 0, 11 and 9 linings of the mandibular cysts, respectively. In situ hybridization revealed the expression of CK18-mRNA in 9 and 4 linings of 24 maxillary and 13 mandibular cysts examined, respectively. With RT-PCR, we explored that both CK18- and CK13-mRNA were expressed not only in the normal nasal and gingival epithelia but also in the examined maxillary cyst linings although their expression levels differed correlating with the difference in CK staining. CONCLUSION: It is concluded that CK13- and CK18-mRNA are constitutively expressed in columnar and squamous epithelial cells, respectively, and that the variant CK expression patterns with CK18-mRNA expression in maxillary radicular cysts are indicative of the possibility of phenotypic transformation in the cyst linings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text