Molecular polymorphism related to flowering trait variation in a Phaseolus vulgaris L. collection
| Autor: | Carlo Tissi, Andrea Mazzucato, Lorenzo Raggi, Valeria Negri |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Candidate gene DNA Plant Genotype Photoperiod MADS Domain Proteins Single-nucleotide polymorphism VRN1 gene Flowers Plant Science Genes Plant Polymorphism Single Nucleotide 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Phaseolus vulgaris L marker-trait associations Genetics Inflorescence Association mapping Plant Proteins 030304 developmental biology Phaseolus 0303 health sciences biology Haplotype food and beverages flowering time General Medicine Vernalization biology.organism_classification Phaseolus vulgaris L. flowering time marker-trait associations candidate genes VRN1 gene Europe Phenotype Haplotypes Gene pool Americas candidate genes Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Science |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2013.11.001 |
| Popis: | The aim of this study was to investigate the flowering variation and the molecular polymorphism in key regulatory genes that control flowering in a Phaseolus vulgaris L. collection of 94 accessions from Europe and the Americas. The analysis of variance revealed that the difference in days-to-flowering between accessions was significant, with European accessions characterized by flowering precocity. Population structure analysis corroborated previous data on the genetic distinction between the Andean and Mesoamerican gene pools. A low level of admixture was detected. Genomic sequences of 15 gene fragments were obtained. About 7.0 kb per accession were sequenced and a total of 48 nucleotide substitutions identified. A Mixed Linear Model analysis, including population structure and kinship, was used to identify marker-trait associations. Haplotype tagging single nucleotide polymorphisms (htSNPs) associated with the studied traits were detected: in PvVRN1 and PvPHYB with days-to-flowering, in PvMYB29 with number of flower buds per inflorescence and in PvTFL1z and PvFCA with inflorescence length. The two genes associated with days-to-flowering control belong to the photoperiod and vernalization pathways. In particular, the PvVRN1 gene appears to play an important role in regulating the adaptation process of common bean. |
| Databáze: | OpenAIRE |
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