CD27 - CD45 + γδ T cells can be divided into two populations, CD27 - CD45 int and CD27 - CD45 hi with little proliferation potential
| Autor: | Kosuke Odaira, Takuya Takahashi, Kazuhiro Kakimi, Nao Fujieda, Hirokazu Matsushita, Shin-nosuke Kimura, Kaori Kambara, Yukari Kobayashi, Izumi T |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
T-Lymphocytes T cell Biophysics chemical and pharmacologic phenomena Biology Biochemistry 03 medical and health sciences Interleukin 21 Immune system Antigen immune system diseases medicine Humans Cytotoxic T cell IL-2 receptor Molecular Biology Cells Cultured Cell Proliferation T-cell receptor virus diseases Receptors Antigen T-Cell gamma-delta hemic and immune systems Cell Biology Phenotype Tumor Necrosis Factor Receptor Superfamily Member 7 030104 developmental biology medicine.anatomical_structure Immunology Leukocyte Common Antigens |
| Zdroj: | Biochemical and Biophysical Research Communications. 478:1298-1303 |
| ISSN: | 0006-291X |
| Popis: | In addition to the majority of T cells which carry the αβ T cell receptor (TCR) for antigen, a distinct subset of about 1-5% of human peripheral blood T cells expressing the γδ TCR contributes to immune responses to infection, tissue damage and cancer. T cells with the Vδ2(+) TCR, usually paired with Vγ9, constitute the majority of these γδ T cells. Analogous to αβ T cells, they can be sorted into naive (CD27(+)CD45RA(+)), central memory (CD27(+)CD45RA(-)), effector memory (CD27(-)CD45RA(-)), and terminally-differentiated effector memory (CD27(-)CD45RA(+)) phenotypes. Here, we found that CD27(-)CD45RA(+) γδ T cells can be further divided into two populations based on the level of expression of CD45RA: CD27(-)CD45RA(int) and CD27(-)CD45RA(hi). Those with the CD27(-)CD45RA(hi) phenotype lack extensive proliferative capacity, while those with the CD27(-)CD45RA(int) phenotype can be easily expanded by culture with zoledronate and IL-2. These CD27(-)CD45RA(hi) potentially exhausted γδ T cells were found predominantly in cancer patients but also in healthy subjects. We conclude that γδ T cells can be divided into at least 5 subsets enabling discrimination of γδ T cells with poor proliferative capacity. It was one of our goals to predict the feasibility of γδ T cell expansion to sufficient amounts for adoptive immunotherapy without the necessity for conducting small-scale culture tests. Fulfilling the ≥1.5% criterion for γδ T cells with phenotypes other than CD27(-)CD45RA(hi), may help avoid small-scale culture testing and shorten the preparation period for adoptive γδ T cells by 10 days, which may be beneficial for patients with advanced cancer. |
| Databáze: | OpenAIRE |
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