Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups
| Autor: | Michel Tibayrenc, Christian Barnabé, Marie-France Bosseno, Simone Frédérique Brenière, Brigitte Bastrenta |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine Satellite DNA lcsh:RC955-962 Lineage (evolution) Trypanosoma cruzi TECHNIQUE RFLP lcsh:QR1-502 satellite DNA DNA Satellite TAXONOMIE DNA sequencing lcsh:Microbiology GENETIQUE DE POPULATION Phylogenetics POLYMORPHISME ENZYMATIQUE ADN SATELLITE parasitic diseases Animals Humans PHYLOGENIE 195 bp repeat unit PARASITE Phylogeny Genetics biology Phylogenetic tree biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD MALADIE DE CHAGAS Restriction fragment length polymorphism ANALYSE GENETIQUE Polymorphism Restriction Fragment Length Microsatellite Repeats |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 94, Iss 3, Pp 323-328 (1999) Memórias do Instituto Oswaldo Cruz, Volume: 94, Issue: 3, Pages: 323-328, Published: MAY 1999 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of #Trypanosoma cruzi$ was analyzed among 23 #T. cruzi$ stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf1 and Hae3 were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf 1) and high molecular weight DNA (Hae 3), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by izoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in #T. cruzi$. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation. (Résumé d'auteur) |
| Databáze: | OpenAIRE |
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