Microbiota of Severe Early Childhood Caries before and after Therapy
| Autor: | C. V. Hughes, Ralph Kent, Anne C.R. Tanner, P. Lif Holgerson, Eleni Kanasi, Ingegerd Johansson, Cheen Y. Loo, N I Chalmers |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Streptococcus parasanguinis Slackia exigua Dental Plaque Prevotella macromolecular substances Dental Caries Gram-Positive Bacteria Dental plaque Polymerase Chain Reaction Microbiology Diet Cariogenic Streptococcus mutans Bacteria Anaerobic Recurrence medicine Humans Least-Squares Analysis Child General Dentistry Oligonucleotide Array Sequence Analysis Bifidobacterium biology Research Reports biology.organism_classification medicine.disease Bacterial Typing Techniques Bifidobacteriaceae stomatognathic diseases Case-Control Studies Child Preschool Early childhood caries Follow-Up Studies |
| Zdroj: | Journal of Dental Research. 90:1298-1305 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034511421201 |
| Popis: | Severe early childhood caries (ECC) is difficult to treat successfully. This study aimed to characterize the microbiota of severe ECC and evaluate whether baseline or follow-up microbiotas are associated with new lesions post-treatment. Plaque samples from 2- to 6-year-old children were analyzed by a 16S rRNA-based microarray and by PCR for selected taxa. Severe-ECC children were monitored for 12 months post-therapy. By microarray, species associated with severe-ECC (n = 53) compared with caries-free (n = 32) children included Slackia exigua (p = 0.002), Streptococcus parasanguinis (p = 0.013), and Prevotella species (p < 0.02). By PCR, severe-ECC-associated taxa included Bifidobacteriaceae (p < 0.001), Scardovia wiggsiae (p = 0.003), Streptococcus mutans with bifidobacteria (p < 0.001), and S. mutans with S. wiggsiae (p = 0.001). In follow-up, children without new lesions (n = 36) showed lower detection of taxa including S. mutans, changes not observed in children with follow-up lesions (n = 17). Partial least-squares modeling separated the children into caries-free and two severe-ECC groups with either a stronger bacterial or a stronger dietary component. We conclude that several species, including S. wiggsiae and S. exigua, are associated with the ecology of advanced caries, that successful treatment is accompanied by a change in the microbiota, and that severe ECC is diverse, with influences from selected bacteria or from diet. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|