Pleiotrophin Expression during Odontogenesis
| Autor: | Amena Tamkenath, Katherine Stidham, Heidi Erlandsen, Mary Elizabeth Wilson, Mary MacDougall, Jennifer E. Ames, Thomas F. Deuel, Olga A. Mamaeva, Pablo Perez-Pinera |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Chemokine
Mesoderm Histology Bone Morphogenetic Protein 7 medicine.medical_treatment Bone Morphogenetic Protein 2 Bone Morphogenetic Protein 4 Pleiotrophin Cell Line Mice stomatognathic system Ameloblasts medicine Animals RNA Messenger Dental Pulp Odontoblasts biology Reverse Transcriptase Polymerase Chain Reaction Growth factor Osteoblast Articles Immunohistochemistry Molar Molecular biology Cell biology Incisor Mice Inbred C57BL Bone morphogenetic protein 7 medicine.anatomical_structure Animals Newborn Bone morphogenetic protein 4 biology.protein Cytokines Anatomy Carrier Proteins Ameloblast Tooth |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 60:366-375 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/0022155412439316 |
| Popis: | Pleiotrophin (PTN) is an extracellular matrix–associated growth factor and chemokine expressed in mesodermal and ectodermal cells. It plays an important role in osteoblast recruitment and differentiation. There is limited information currently available about PTN expression during odontoblast differentiation and tooth formation, and thus the authors aimed to establish the spatiotemporal expression pattern of PTN during mouse odontogenesis. Immortalized mouse dental pulp (MD10-D3, MD10-A11) and odontoblast-like (M06-G3) and ameloblast-like (EOE-3M) cell lines were grown and samples prepared for immunocytochemistry, Western blot, and conventional and quantitative PCR analysis. Effects of BMP2, BMP4, and BMP7 treatment on PTN expression in odontoblast-like M06-G3 cells were tested by quantitative PCR. Finally, immunohistochemistry of sectioned mice mandibles and maxillaries at developmental stages E16, E18, P1, P6, P10, and P28 was performed. The experiments showed that PTN, at both the mRNA and protein level, was expressed in all tested epithelial and mesenchymal dental cell lines and that the level of PTN mRNA was influenced differentially by the bone morphogenetic proteins. The authors observed initial expression of PTN in the inner enamel epithelium with prolonged expression in the ameloblasts and odontoblasts throughout their stages of maturation and strong expression in the terminally differentiated and enamel matrix–secreting ameloblasts and odontoblasts of the adult mouse incisors and molars. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|